[在连续流动12通道分析仪中进行全酶法甘油三酯测定（作者译）]

[A fully enzymatic triglyceride determination in a continuous flow 12-channel analyzer (author's transl)].

作者信息

Liebich H M, Müller P H, Schmülling R M, Eggstein M, Stähler F, Weisshaar D

出版信息

J Clin Chem Clin Biochem. 1977 Sep;15(9):503-8.

Abstract

A fully enzymatic triglyceride determination utilizing enzymatic hydrolysis with a lipase-esterase mixture and subsequent enzymatic glycerol determination, has been adapted for use in a continuous flow 12-channel-analyzer. The method is linear up to 7.9 mmol/l (700 mg/100 ml). The analytical precision in the concentration range of 1.5 to 5.4 mmol/l (133 to 478 mg/100 ml) is characterized by relative standard deviations of 0.8 to 4.8%. In the lower measuring range at concentrations around 0.7 mmol/l (62 mg/100 ml) a mean relative standard deviation of 7.2% is found for 1140 measurements under routine conditions. For triglyceride concentrations of 0.9 to 7.7 mmol/80 to 680 mg/100 ml) a mean relative coefficient of verspill Q = 2.0 is determined. Bilirubin caused no observable interference in the determination. In comparison with the manual method by Eggstein & Kreutz (1966) Klin. Wochenschr. 44, 262-267) the results from the fully enzymatic method on the 12-channel-analyzer were lower by approximately 16%, corrected by an additive factor of 0.029 mmol/l (2.59 mg/100 ml). The accuracy controls with controls sera showed a difference of 10%.

摘要

一种利用脂肪酶 - 酯酶混合物进行酶促水解并随后测定酶促甘油的全酶法甘油三酯测定方法，已被应用于连续流动12通道分析仪。该方法在高达7.9 mmol/l（700 mg/100 ml）时呈线性。在1.5至5.4 mmol/l（133至478 mg/100 ml）浓度范围内的分析精密度，其相对标准偏差为0.8%至4.8%。在常规条件下，对于浓度约为0.7 mmol/l（62 mg/100 ml）的较低测量范围，1140次测量的平均相对标准偏差为7.2%。对于甘油三酯浓度为0.9至7.7 mmol/80至680 mg/100 ml），测定平均相对溢出系数Q = 2.0。胆红素在测定中未产生明显干扰。与Eggstein和Kreutz（1966年，《临床周刊》44, 262 - 267）的手工方法相比，12通道分析仪上全酶法的结果低约16%，通过添加0.029 mmol/l（2.59 mg/100 ml）的校正因子进行校正。用对照血清进行的准确度控制显示差异为10%。