Ekindjian O G, Duchassaing D
Ann Biol Clin (Paris). 1979;37(3):175-80.
The authors describe a critical study of two entirely enzymatic methods of determination of serum triglycerides which differ mainly by the choice of hydrolytic agents: esterase and hydrolase by the Roche methods, lipase and esterase in the case of the Boehringer method. The estimation of the resulting free glycerol requires 3 sequential enzymatic reactions (glycerol kinase, pyruvate kinase, lactate dehydrogenase, ending by the measurement of the consumed reduced NAD. The study of the kinetics of the analysis in two stages is carried out using standard solutions of glycerol and with human sera of variable concentration; the optimal conditions of the reaction time and temperature are drawn up. The trials of repeatability and reproducibility from day to day were carried out together with evaluation of the precision. The method was compared with Beohringer's method (n = 50) using hydrolysis of alcoholic potash. The authors conclude on the use of the methods studied.
作者描述了一项对两种完全酶法测定血清甘油三酯的关键研究，这两种方法主要因水解剂的选择而不同：罗氏方法采用酯酶和水解酶，而勃林格方法则使用脂肪酶和酯酶。对生成的游离甘油的估算需要3个连续的酶促反应（甘油激酶、丙酮酸激酶、乳酸脱氢酶，最后通过测量消耗的还原型烟酰胺腺嘌呤二核苷酸来完成）。分两个阶段对分析动力学进行研究，使用甘油标准溶液以及不同浓度的人血清；确定了反应时间和温度的最佳条件。进行了日复一日的重复性和再现性试验，并对精密度进行了评估。该方法与使用氢氧化钾酒精溶液水解的勃林格方法（n = 50）进行了比较。作者对所研究方法的应用得出了结论。
