Hafkenscheid J C, Dijt C C
J Clin Chem Clin Biochem. 1977 Sep;15(9):519-21. doi: 10.1515/cclm.1977.15.1-12.519.
A comparison was made between the aminotransferase activities of a number of plasma or serum samples in 4 reaction media: phosphate buffer, phosphate buffer + pyridoxal-5'-phosphate, tris buffer and tris buffer + pyridoxal-5'-phosphate. The reactions were carried out in the various buffer systems on the same day at 35 degrees C on an automatic kinetic enzyme system (AKES, Vitatron, Dieren, the Netherlands). The highest enzymic activity of both aminotransferases is observed in tris buffer + pyridoxal-5'-phosphate. The activity is about 10--15% higher than in phosphate buffer + pyridoxal-5'-phosphate. Without supplementary pyridoxal-5'-phosphate the differences between both buffer systems are lower or absent. It appears necessary, therefore, to determine the activity of both aminotransferases in a buffer system with tris and to add pyridoxal-5'-phosphate, since assays should be carried out in the presence of optimal concentrations of any of the necessary factors.
对多种血浆或血清样本在4种反应介质中的转氨酶活性进行了比较：磷酸盐缓冲液、磷酸盐缓冲液+磷酸吡哆醛、三羟甲基氨基甲烷缓冲液和三羟甲基氨基甲烷缓冲液+磷酸吡哆醛。这些反应于同一天在35摄氏度下，在自动动力学酶系统（AKES，Vitatron，荷兰迪伦）上，在各种缓冲系统中进行。在三羟甲基氨基甲烷缓冲液+磷酸吡哆醛中观察到两种转氨酶的酶活性最高。该活性比在磷酸盐缓冲液+磷酸吡哆醛中高约10%-15%。在没有补充磷酸吡哆醛的情况下，两种缓冲系统之间的差异较小或不存在。因此，似乎有必要在含有三羟甲基氨基甲烷的缓冲系统中测定两种转氨酶的活性，并添加磷酸吡哆醛，因为测定应在任何必要因素的最佳浓度存在下进行。
