Khongwir B S, Dani H M, Dhanda J S
Department of Biochemistry, Panjab University, Chandigarh, India.
J Environ Pathol Toxicol Oncol. 1997;16(1):27-31.
Microsomal preparations from rats treated with 2-acetylaminofluorene (2-AAF) or with 4-dimethylaminoazobenzene (4-DAB), alone or followed by phenobarbital (PB), showed almost complete loss of microsomal proteins of molecular weights higher than 60,000, as shown by Sephadex G-150 gel filtration technique. Induction of a number of microsomal proteins in the lower ranges of molecular weights was also recorded due to the treatment of these two hepatocarcinogens with and without the use of PB as a promoter. These modifications in the protein patterns of microsomes might be due to altered genetic expression resulting in uncontrolled cell division/cell cycle.
用2-乙酰氨基芴（2-AAF）或4-二甲基氨基偶氮苯（4-DAB）单独处理大鼠，或在处理后再用苯巴比妥（PB）处理，其微粒体制剂经葡聚糖凝胶G-150凝胶过滤技术显示，分子量高于60,000的微粒体蛋白几乎完全丧失。无论是否使用PB作为启动剂，用这两种肝癌致癌物处理后，还记录到一些分子量较低的微粒体蛋白的诱导情况。微粒体蛋白质模式的这些改变可能是由于基因表达改变导致细胞分裂/细胞周期失控所致。
