Dente F L, Carnevali S, Paggiaro P L, Cianchetti S, Bacci E, Bancalari L, Di Franco A, Giannini D, Vagaggini B, Giuntini C
Second Institute of Internal Medicine, University of Pisa, Italy.
Respiration. 1997;64(4):285-90. doi: 10.1159/000196688.
In order to evaluate the relationship between allergen-induced heat-stable neutrophil chemotactic activity (HS-NCA) release during early asthmatic reaction (EAR) and the presence of a late asthmatic reaction (LAR), serum HS-NCA was measured at three serum dilutions (1:5, 1:40, 1:200) during EAR induced by allergen in 26 atopic asthmatics, 13 with isolated EAR and 13 with EAR followed by LAR. HS-NCA was measured using a 48-well microchamber with 5-micron-pore-size nitrocellulose filters, using isolated neutrophils from healthy donors and the leading front technique. Subjects with LAR developed EAR after inhalation of a lower dose of allergen than subjects with isolated EAR. Increase in serum HS-NCA during EAR was significantly higher in subjects with isolated EAR than in subjects with EAR plus LAR at the 1:5 dilution, while it was significantly higher in subjects with EAR plus LAR than in the subjects with isolated EAR at the 1:200 dilution; the 1:40 dilution gave similar results in both groups. Changes in serum HS-NCA during EAR significantly correlated with the maximum decrease in forced expiratory volume in 1 s (FEV1) during LAR: a higher decrease in FEV1 during LAR was associated with a lower increase in HS-NCA at the 1:5 dilution (Spearman's rho = 0.43, rho = 0.03), and with a higher increase in NCA at the 1:200 dilution (Spearman's p = -0.46, p = 0.02). These results can be explained by the 'high-dose-inhibition' phenomenon. Assuming that HS-NCA is associated with mast cell degranulation in the airways after allergen challenge, these findings demonstrate that higher mast cell activation during EAR is present in subjects with a subsequent LAR than in subjects with isolated EAR.
为了评估变应原诱导的早期哮喘反应（EAR）期间热稳定中性粒细胞趋化活性（HS-NCA）释放与迟发性哮喘反应（LAR）存在之间的关系，在26例特应性哮喘患者中，于变应原诱导的EAR期间，在三种血清稀释度（1:5、1:40、1:200）下测量血清HS-NCA，其中13例仅有EAR，13例EAR后伴有LAR。使用带有5微米孔径硝酸纤维素滤膜的48孔微室，采用来自健康供体的分离中性粒细胞和前沿技术测量HS-NCA。与仅有EAR的受试者相比，伴有LAR的受试者在吸入较低剂量变应原后出现EAR。在1:5稀释度时，仅有EAR的受试者在EAR期间血清HS-NCA的增加显著高于EAR加LAR的受试者，而在1:200稀释度时，EAR加LAR的受试者血清HS-NCA的增加显著高于仅有EAR的受试者；1:40稀释度时两组结果相似。EAR期间血清HS-NCA的变化与LAR期间1秒用力呼气量（FEV1）的最大下降显著相关：LAR期间FEV1下降越高，在1:5稀释度时HS-NCA的增加越低（Spearman相关系数ρ = 0.43，P = 0.03），而在1:200稀释度时NCA的增加越高（Spearman相关系数p = -0.46，P = 0.02）。这些结果可用“高剂量抑制”现象来解释。假设HS-NCA与变应原激发后气道中的肥大细胞脱颗粒有关，这些发现表明，与仅有EAR的受试者相比，随后伴有LAR的受试者在EAR期间存在更高的肥大细胞活化。
