Adu F D, Omotade O O, Ikusika O, Oyedele I O
Department of Virology and Institute of Child Health, College of Medicine, University of Ibadan.
West Afr J Med. 1997 May-Jun;16(2):109-11.
We have recently succeeded in isolating two measles viruses, Ibn H13153 and Ibn H13154 from the peripheral lymphocytes of two unvaccinated 9 and 12 month old outpatient children at the Institute of Child Health, University College Hospital Ibadan, Nigeria. The viruses have undergone six serial passages in B95a and Vero cell lines where characteristic measles cytopathic effects (CPE) have been noticed. The viruses have been confirmed to be measles by Haemagglutination Inhibition (HI) test and the Indirect Immunofluorescent antibody Test (IFT). Both isolates agglutinated 0.75% monkey rbc. The HA titres were 1:40 and 1:80 respectively. Antigens from the known positive Edmonston strain and uninoculated Vero cell line gave an HA titre of 1:160 and 1:0 respectively. Further characterisation with respect to the polypeptide and DNA components is under way.
我们最近成功地从尼日利亚伊巴丹大学学院医院儿童健康研究所两名未接种疫苗的9个月和12个月大门诊儿童的外周淋巴细胞中分离出两种麻疹病毒，即伊本H13153和伊本H13154。这些病毒已在B95a和Vero细胞系中连续传代六次，在这些细胞系中观察到了典型的麻疹细胞病变效应（CPE）。通过血凝抑制（HI）试验和间接免疫荧光抗体试验（IFT）已确认这些病毒为麻疹病毒。两种分离株均能凝集0.75%的猴红细胞。血凝素（HA）滴度分别为1:40和1:80。已知阳性的埃德蒙斯顿毒株和未接种的Vero细胞系的抗原血凝素滴度分别为1:160和1:0。关于多肽和DNA成分的进一步鉴定正在进行中。
