Wolfe R G, Maxwell C V, Nelson E C, Johnson R R
J Nutr. 1977 Dec;107(12):2100-8. doi: 10.1093/jn/107.12.2100.
Thirty male neonatal pigs were obtained by cesarean section and randomly allotted from littermate groups to three diets containing 2%, 17%, and 32% fat on a dry matter basis. Butterfat was used to replace glucose in the isoenergetic liquid, semipurified diets. Each pig was placed in a sterile isolator and fed ad libitum five times daily. After 14 days, pigs were weighed, killed, and samples of liver and backfat were obtained. The carcass of each pig was ground and samples obtained for determination of total body fat and protein. An increase in the level of dietary fat resulted in a significant increase in 14 day weight gain and a tendency for improved feed efficiency. These results demonstrate not only that the neonatal pig can utilize semipurified liquid diets high in butterfat content, but also that energy from butterfat appears to be used as efficiently as energy from glucose from growth purposes. Increasing dietary fat level decreased the activity of fatty acid synthetase and citrate cleavage enzyme in adipose tissue and liver, and decreased the activity of malic enzyme in adipose tissue. The specific activities of these three enzymes were higher in adipose tissue than in liver.
通过剖腹产获得30只雄性新生仔猪，并从同窝仔猪组中随机分配到三种干物质基础含脂量分别为2%、17%和32%的日粮中。在等能量的液体半纯化日粮中，用乳脂肪替代葡萄糖。将每头仔猪置于无菌隔离器中，每天自由采食5次。14天后，对仔猪称重、处死后，采集肝脏和背膘样本。将每头仔猪的胴体研磨，采集样本用于测定体脂和蛋白质总量。日粮脂肪水平的提高导致14天体重显著增加，且饲料效率有提高的趋势。这些结果不仅表明新生仔猪能够利用乳脂肪含量高的半纯化液体日粮，而且从生长目的来看，乳脂肪中的能量似乎与葡萄糖中的能量利用效率一样高。提高日粮脂肪水平会降低脂肪组织和肝脏中脂肪酸合成酶和柠檬酸裂解酶的活性，以及脂肪组织中苹果酸酶的活性。这三种酶的比活性在脂肪组织中高于肝脏。
