MacKinnon M G, Drebot M A, Tyrrell G J
Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
Antimicrob Agents Chemother. 1997 Aug;41(8):1805-7. doi: 10.1128/AAC.41.8.1805.
The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.
耐万古霉素肠球菌的vanY基因编码一种D，D-羧肽酶。通过使用聚合酶链反应（PCR）检测策略，发现一株VanA粪肠球菌临床分离株在vanY中有一个名为IS1476的插入序列（IS）样元件。在以二乙酰-L-赖氨酸-D-丙氨酸-D-丙氨酸为底物的荧光邻苯二甲醛测定中，该分离株中VanY D，D-羧肽酶的活性降低。据我们所知，这是首次在万古霉素抗性基因中报道IS样元件。
