Chang W, Waltenbaugh C, Borensztajn J
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611, USA.
Metabolism. 1997 Aug;46(8):926-9. doi: 10.1016/s0026-0495(97)90081-0.
Fatty acid ethyl esters (FAEEs), nonoxidative by-products of ethanol metabolism, are found in various tissues and plasma after ethanol ingestion and may be responsible for some of the pathological changes observed in alcohol-consuming individuals. Previous studies demonstrated that several different enzymes, including lipoprotein lipase (LPL), can catalyze FAEE synthesis in vitro. We report that LPL catalyzes FAEE synthesis in isolated rat hearts perfused with chylomicrons in the presence of ethanol. Most of the FAEEs accumulated in the perfusate, suggesting that in vivo, plasma FAEEs derive from LPL-mediated synthesis. Our results are the first demonstration of the direct involvement of a specific enzyme, LPL, in FAEE synthesis under physiological conditions.
脂肪酸乙酯（FAEEs）是乙醇代谢的非氧化副产物，在摄入乙醇后可在各种组织和血浆中发现，可能是饮酒个体中观察到的一些病理变化的原因。先前的研究表明，包括脂蛋白脂肪酶（LPL）在内的几种不同酶可在体外催化FAEE合成。我们报告称，在有乙醇存在的情况下，LPL可在灌注乳糜微粒的离体大鼠心脏中催化FAEE合成。大多数FAEEs积聚在灌注液中，这表明在体内，血浆FAEEs来源于LPL介导的合成。我们的结果首次证明了一种特定酶LPL在生理条件下直接参与FAEE合成。
