从渗透调节表达系统中纯化活性大肠杆菌核糖体循环因子（RRF）。
Purification of active Escherichia coli ribosome recycling factor (RRF) from an osmo-regulated expression system.
作者信息
MacDougall J, Holst-Hansen P, Mortensen K K, Freistroffer D V, Pavlov M Y, Ehrenberg M, Buckingham R H
机构信息
CNRS-UPR 9073, Institut de Biologie Physico-chimique, Paris, France.
出版信息
Biochimie. 1997 May;79(5):243-6. doi: 10.1016/s0300-9084(97)83511-0.
Ribosome release factor (RRF) from Escherichia coli was overproduced from an osmo-expression vector. More than 40% of cell protein was RRF after 6 h of induction. A purification scheme is described that produced 50 mg of RRF from an initial culture of 2 L. The recycling time for ribosomes synthesising the tripeptide fMet-Phe-Leu in vitro in the absence of RF3 was reduced from 40 to 15 s by the addition of purified 1.5 microM RRF.
来自大肠杆菌的核糖体释放因子（RRF）通过渗透压表达载体过量表达。诱导6小时后，超过40%的细胞蛋白为RRF。描述了一种纯化方案，该方案从2升初始培养物中产生了50毫克RRF。在没有RF3的情况下，通过添加纯化的1.5微摩尔RRF，体外合成三肽fMet-Phe-Leu的核糖体的循环时间从40秒减少到15秒。
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