Zhang L, Roth G S
Molecular Physiology & Genetics Section, NIA/NIH, Baltimore, MD 21224, USA.
Neurobiol Aging. 1997 Mar-Apr;18(2):251-5. doi: 10.1016/s0197-4580(97)00011-0.
In situ hybridization of a digoxigenin-labeled oligonucleotide probe combined with computer assisted image assessment was used to directly visualize D1 dopamine receptor mRNA-containing neurons in rat striata, and quantify age-related changes in the sizes and relative mRNA content of these neurons. It was found that 1) numbers of D1 mRNA-containing neurons appear to decrease in striata of aged rats; 2) relative amounts of D1 receptor mRNA in individual striatal neurons do not change during aging; and 3) neurons of all sizes appear to be lost, with the greatest absolute decrease in those larger than 150 microns2. These data confirm the age associated loss of D1 dopamine receptors reported for some other rat strains in that species, and further suggest that neuronal loss is responsible for the decrement. The latter finding contrasts with the aging associated loss of D2 dopamine receptors, which additionally involves decreased amounts of mRNA per cell.
采用地高辛配体标记的寡核苷酸探针原位杂交结合计算机辅助图像评估，直接观察大鼠纹状体中含D1多巴胺受体mRNA的神经元，并量化这些神经元大小和相对mRNA含量的年龄相关变化。结果发现：1）老年大鼠纹状体中含D1 mRNA的神经元数量似乎减少；2）单个纹状体神经元中D1受体mRNA的相对含量在衰老过程中不变；3）各种大小的神经元似乎都有丢失，其中大于150平方微米的神经元绝对减少最多。这些数据证实了该物种中其他一些大鼠品系所报道的与年龄相关的D1多巴胺受体丢失，进一步表明神经元丢失是导致这种减少的原因。后一发现与D2多巴胺受体随年龄增长的丢失形成对比，后者还涉及每个细胞中mRNA含量的减少。
