Munson J W, Bilous R
J Pharm Sci. 1977 Oct;66(10):1403-5. doi: 10.1002/jps.2600661013.
A colorimetric method for the determination of carboxylic acids based on the dicyclohexylcarbodiimide-coupled reaction of 2-nitrophenylhydrazine and carboxylic acids is described. The product of the reaction is extracted into aqueous sodium hydroxide to produce a blue color. This method is suitable for the analysis of aliphatic acids, but aromatic acids do not react under these conditions.
描述了一种基于2-硝基苯肼与羧酸的二环己基碳二亚胺偶联反应测定羧酸的比色法。反应产物用氢氧化钠水溶液萃取，产生蓝色。该方法适用于脂肪酸的分析，但芳香酸在这些条件下不发生反应。
