用葡萄糖氧化酶法测定尿中氯噻酮水平。

GLC determination of urinary chlorthalidone levels.

作者信息

Li H, Johnston M M, Mufson D

出版信息

J Pharm Sci. 1977 Dec;66(12):1732-4. doi: 10.1002/jps.2600661219.

Abstract

A sensitive and specific GLC method for the determination of urinary chlorthalidone levels was developed using on-column methylation. Chlorthalidone is converted to a tetramethylated derivative with trimethylanilinium hydroxide in methanol. This method which permits the determination of as little as 0.1 microgram of chlorthalidone/ml of dog urine, should be adequate for use with human subjects receiving a clinical dose.

摘要

采用柱上甲基化法建立了一种灵敏且特异的气相色谱法，用于测定尿中氯噻酮水平。氯噻酮在甲醇中与氢氧化三甲基苯胺反应转化为四甲基化衍生物。该方法能够测定低至0.1微克/毫升犬尿中的氯噻酮，适用于接受临床剂量的人类受试者。