Mannik M, Wener M H
University of Washington, Seattle 98195-6428, USA.
Arthritis Rheum. 1997 Aug;40(8):1504-11. doi: 10.1002/art.1780400819.
To determine if antibodies to the collagen-like region of C1q (C1q-CLR) are present in the glomerular immune deposits of patients with systemic lupus erythematosus (SLE).
Kidney tissues were obtained at autopsy, glomeruli were isolated, and glomerular basement membrane fragments were prepared. Antibodies were extracted with low pH or with DNase.
The concentrations of antibodies to C1q-CLR recovered from the glomeruli were > or =50-fold higher per unit of IgG than that found in the serum or in the serum and interstitial fluid entrained in glomeruli. Antibodies to C1q-CLR were recovered from glomeruli of 4 of 5 patients with proliferative glomerulonephritis at autopsy.
This is the first demonstration that antibodies to C1q-CLR are deposited and concentrated in the renal glomeruli of patients with SLE. These antibodies, thus, have the potential of contributing to the pathogenesis of lupus glomerulonephritis.
确定系统性红斑狼疮（SLE）患者肾小球免疫沉积物中是否存在针对C1q胶原样区域（C1q-CLR）的抗体。
在尸检时获取肾脏组织，分离肾小球，并制备肾小球基底膜片段。用低pH值或脱氧核糖核酸酶提取抗体。
从肾小球中回收的针对C1q-CLR的抗体浓度，每单位IgG比血清中或肾小球中夹带的血清和间质液中的浓度高50倍或更高。在尸检时，5例增殖性肾小球肾炎患者中有4例的肾小球中回收了针对C1q-CLR的抗体。
这是首次证明针对C1q-CLR的抗体沉积并浓缩于SLE患者的肾小球中。因此，这些抗体有可能参与狼疮性肾小球肾炎的发病机制。
