Murrin L C, Enna S J, Kuhar M J
J Pharmacol Exp Ther. 1977 Dec;203(3):564-74.
[3H]Reserpine was administered (i.v.) to rats and they were killed 7 days later. At this time, the regional localization of radioactivity paralleled the distribution of specific binding sites observed in other laboratories. Autoradiographic studies of certain areas displayed a striking localization of radioactivity. There was a marked association of autoradiographic grains with areas containing catecholamine systems. In particular, the locus ceruleus, the caudate-putamen, the nucleus accumbens, the dorsolateral septum and the infundibulum had high grain densities. In the caudateputamen, there was a clear localization of grains to the neuropil. There was also a striking association of autoradiographic grains with certain hypothalamic nuclear, i.e., the dorsal premammillary nucleus, the prelateral mammillary nucleus and the lateral mammillary nucleus. In these areas, the grains were clearly localized in the cytoplasm of the cell bodies. All of the above localizations of autoradiographic grains were blocked by administration of unlabeled reserpine before injection of [3H]reserpine. The significance of these findings and their relationship to the clinical actions of reserpine are discussed.
给大鼠静脉注射[3H]利血平，7天后将其处死。此时，放射性的区域定位与其他实验室观察到的特异性结合位点分布平行。对某些区域的放射自显影研究显示出放射性的显著定位。放射自显影颗粒与含有儿茶酚胺系统的区域有明显关联。特别是，蓝斑、尾状核-壳核、伏隔核、背外侧隔区和漏斗部有高颗粒密度。在尾状核-壳核中，颗粒明显定位于神经毡。放射自显影颗粒与某些下丘脑核，即背内侧乳头前核、外侧乳头前核和外侧乳头核也有显著关联。在这些区域，颗粒明显定位于细胞体的细胞质中。在注射[3H]利血平之前给予未标记的利血平，可阻断上述所有放射自显影颗粒的定位。讨论了这些发现的意义及其与利血平临床作用的关系。
