Lown J A, Ivey J G
Department of Haematology, Royal Perth Hospital, Western Australia.
Transfus Med. 1991 Sep;1(3):163-7. doi: 10.1111/j.1365-3148.1991.tb00026.x.
Solid-phase red-cell adherence (SPRCA) techniques in platelet serology are used mainly for crossmatching. A SPRCA method for general diagnostic application was evaluated in parallel with the platelet suspension immunofluorescence test (PIFT). Of 149 patient sera sent for investigation of thrombocytopaenia, 76 were negative and 59 positive when studied by both methods, eight positive by PIFT only and six positive by SPRCA only. The reactivity observed for 24 sera containing HLA antibodies tested with chloroquine-treated and untreated platelets was similar for both methods. All of 14 sera containing quinine-associated antibodies reacted strongly to both techniques in the presence of added quinine. In comparison, however, whereas all sera were nonreactive to SPRCA in the absence of added quinine, and with PIFT, seven of the sera reacted weakly. Titration studies with three examples of anti-PlA1 and five sera containing HLA antibodies generally showed a one doubling dilution lower titre with the SPRCA procedure. End-point interpretation, however, was more readily achieved with the SPRCA method. The SPRCA technique displays similar sensitivity and specificity to the PIFT and is recommended for use by routine hospital laboratories to screen platelet antibodies.
血小板血清学中的固相红细胞黏附（SPRCA）技术主要用于交叉配血。我们将一种用于一般诊断的SPRCA方法与血小板悬液免疫荧光试验（PIFT）进行了平行评估。在送检血小板减少症调查的149份患者血清中，两种方法检测时76份为阴性，59份为阳性，8份仅PIFT为阳性，6份仅SPRCA为阳性。用氯喹处理和未处理的血小板检测24份含HLA抗体的血清时，两种方法观察到的反应性相似。在添加奎宁的情况下，14份含奎宁相关抗体的血清对两种技术均有强烈反应。然而，相比之下，在未添加奎宁时，所有血清对SPRCA均无反应，而对于PIFT，其中7份血清有微弱反应。用3例抗PlA1和5份含HLA抗体的血清进行滴定研究，结果显示SPRCA法的效价通常低一个稀释倍数。然而，用SPRCA法更容易达到终点判读。SPRCA技术与PIFT显示出相似的敏感性和特异性，推荐医院常规实验室用于筛查血小板抗体。
