Kirschke H, Clausen T, Göhring B, Günther D, Heucke E, Laube F, Löwe E, Neef H, Papesch H, Peinze S, Plehn G, Rebmann U, Rinne A, Rüdrich R, Weber E
Institute of Physiological Chemistry, Martin-Luther University, Halle (Saale), Germany.
J Cancer Res Clin Oncol. 1997;123(7):402-6. doi: 10.1007/BF01240124.
Renal cell carcinoma contains significantly lower concentrations of the lysosomal cysteine proteases, cathepsins B, C, H, L and S, than does normal kidney, as shown by several methods, such as activity determination, enzyme-linked immunosorbent assay, immunoblotting and immunohistochemistry. The same low levels of enzyme activity and concentration have been determined in renal cell carcinoma metastases in the lung. Our results on the decreased concentration of cysteine peptidases at the protein level would seem to conflict with earlier results on an increased concentration of the cathepsin L mRNA in renal cell carcinoma.
几种方法（如活性测定、酶联免疫吸附测定、免疫印迹和免疫组织化学）显示，肾细胞癌中溶酶体半胱氨酸蛋白酶组织蛋白酶B、C、H、L和S的浓度显著低于正常肾脏。在肺肾细胞癌转移灶中也测定到了同样低水平的酶活性和浓度。我们关于蛋白质水平半胱氨酸肽酶浓度降低的结果，似乎与早期关于肾细胞癌中组织蛋白酶L mRNA浓度升高的结果相矛盾。
