Gutiérrez-Ibarluzea I, Mendizabal-Zubiaga J L, Reblet C, Bueno-López J L
Department of Neuroscience, School of Medicine and Dentistry, University of the Basque Country, Leioa, Biscay, Spain.
Neuroreport. 1997 Jul 28;8(11):2495-9. doi: 10.1097/00001756-199707280-00016.
The co-localization of GABA with AMPA receptor subunits GluR1 or GluR2/3 was analysed in the striate cortex of adult rats by post-embedding immunocytochemistry in semithin sections. Adjacent 1 micron semithin sections of four brains were alternately incubated with specific antibodies against GABA and the GluR1 and GluR2/3 subunits. The post-embedding immunocytochemistry showed that 38% of GABAergic neurons contained the GluR1 subunit and 10% contained the GluR2/3 subunits. Previous work has shown GluR1 immunoreactivity in non-pyramidal neurons and GluR2/3 immunoreactivity in pyramidal neurons. However, this study is the first to demonstrate that there are GABAergic neurons co-localized with GluR2/3 AMPA subunits. Additionally, this study provides quantitative estimations of the laminar distribution of GABAergic and non-GABAergic cells containing the AMPA GluR1 and GluR2/3 subunits.
通过半薄切片包埋后免疫细胞化学方法，分析了成年大鼠纹状皮质中GABA与AMPA受体亚基GluR1或GluR2/3的共定位情况。对取自四个大脑的相邻1微米半薄切片，分别交替用抗GABA以及GluR1和GluR2/3亚基的特异性抗体进行孵育。包埋后免疫细胞化学结果显示，38%的GABA能神经元含有GluR1亚基，10%含有GluR2/3亚基。先前的研究表明，非锥体神经元中有GluR1免疫反应性，锥体神经元中有GluR2/3免疫反应性。然而，本研究首次证明存在与GluR2/3 AMPA亚基共定位的GABA能神经元。此外，本研究还对含有AMPA GluR1和GluR2/3亚基的GABA能和非GABA能细胞的层状分布进行了定量估计。
