Titgemeyer E C
Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506-1600, USA.
J Anim Sci. 1997 Aug;75(8):2235-47. doi: 10.2527/1997.7582235x.
A variety of factors influence the results of digestion studies designed to measure ruminal disappearance of nutrients and flow of nutrients to the intestine. Feed intake, marker usage, sample collection, statistical design, and computation methods are among the factors discussed in this review. Guidelines are provided for presentation and interpretation of data from digesta flow studies. The scientific value of such studies often is limited because 1) replication, relative to expected variation, is inadequate and 2) experimental animals are not maintained under conditions similar to those used in normal production. Because studies conducted with inappropriate methods or inadequate replication are of little value and waste resources, validation of techniques is critical, and individual researchers should strive to verify their techniques.
多种因素会影响旨在测定瘤胃营养物质消失率和营养物质向肠道流动情况的消化研究结果。采食量、标记物使用、样本采集、统计设计和计算方法等都是本综述中讨论的因素。本文提供了瘤胃食糜流动研究数据呈现和解读的指导原则。此类研究的科学价值往往有限，原因如下：1）相对于预期变异而言，重复次数不足；2）实验动物并非在与正常生产条件相似的环境下饲养。由于采用不当方法或重复次数不足所开展的研究价值不大且浪费资源，因此技术验证至关重要，每位研究人员都应努力验证自己的技术。
