Emri T, Pócsi I, Szentirmai A
Department of Microbiology and Biotechnology, Kossuth Lajos University, Debrecen, Hungary.
J Basic Microbiol. 1997;37(3):181-6. doi: 10.1002/jobm.3620370306.
Enzymes of the glutathione-dependent detoxification pathway (glutathione S-transferase and gamma-glutamyl-transpeptidase) were induced, and the glutathione pool was completely depleted by phenoxyacetic acid in Penicillium chrysogenum mycelia incubated for 15 h in a culture medium containing lactose as a carbon source and sodium glutamate as a nitrogen source. A significant increase in both the oxidised glutathione concentrations and the glutathione reductase activities were also observed. 1-Chloro-2,4-dinitrobenzene--a potent substrate and inducer of glutathione S-transferase-initiated very similar physiological changes but no beta-lactam production could be detected in this case. When (NH4)2HPO4 was used as a nitrogen source the penicillin biosynthesis was repressed and the induction of gamma-glutamyltranspeptidase by phenoxyacetic acid was hindered considerably.
在以乳糖为碳源、谷氨酸钠为氮源的培养基中培养15小时的产黄青霉菌丝体中，谷胱甘肽依赖性解毒途径的酶（谷胱甘肽S-转移酶和γ-谷氨酰转肽酶）被诱导，并且谷胱甘肽池被苯氧乙酸完全耗尽。同时还观察到氧化型谷胱甘肽浓度和谷胱甘肽还原酶活性均显著增加。1-氯-2,4-二硝基苯——一种强效的谷胱甘肽S-转移酶底物和诱导剂——引发了非常相似的生理变化，但在这种情况下未检测到β-内酰胺的产生。当使用磷酸氢二铵作为氮源时，青霉素生物合成受到抑制，并且苯氧乙酸对γ-谷氨酰转肽酶的诱导也受到很大阻碍。
