Leitch E C, Willcox M D
Cooperative Research Centre for Eye Research and Technology, University of NSW, Sydney, Australia.
Aust N Z J Ophthalmol. 1997 May;25 Suppl 1:S20-2. doi: 10.1111/j.1442-9071.1997.tb01747.x.
As part of the normal microbiota of the external eye, Staphylococcus epidermidis has probably developed strategies to overcome tear defences. The interaction of this bacteria with constitutive tear proteins was examined. Isolates of S. epidermidis grown in 10% serum or artificial tear fluid containing lysozyme or lactoferrin were resistant to the action of these proteins. Using ELISA, cell wall binding of C3, vitronectin and lactoferrin differed quantitatively between strains and in closed-eye compared to open-eye conditions. No differences were observed between ocular and non-ocular strains. This suggests that ocular isolates originate from the general host microbiota and S. epidermidis isolates are resistant to individual constitutive tear proteins.
作为眼外部正常微生物群的一部分，表皮葡萄球菌可能已经形成了克服泪液防御的策略。研究了这种细菌与组成性泪液蛋白的相互作用。在含有溶菌酶或乳铁蛋白的10%血清或人工泪液中生长的表皮葡萄球菌分离株对这些蛋白的作用具有抗性。使用酶联免疫吸附测定法（ELISA），C3、玻连蛋白和乳铁蛋白的细胞壁结合在菌株之间以及闭眼与睁眼条件下存在定量差异。眼部菌株和非眼部菌株之间未观察到差异。这表明眼部分离株源自宿主的一般微生物群，并且表皮葡萄球菌分离株对单个组成性泪液蛋白具有抗性。
