Ozaki T, Kondo K, Nakamura Y, Ichimiya S, Nakagawara A, Sakiyama S
Division of Biochemistry, Chiba Cancer Center Research Institute, 666-2, Nitona, Chuoh-ku, Chiba, 260, Japan.
Biochem Biophys Res Commun. 1997 Aug 18;237(2):245-50. doi: 10.1006/bbrc.1997.7122.
Recently, we have identified a new protein (DA41) which can associate with candidate tumor-suppressor DAN protein. In the present study, we have searched for DA41-interacting protein(s), using a yeast two-hybrid system. An adult rat lung cDNA library was screened by using a truncated form of DA41 (1-308) which lacks a DAN-binding region as bait. One of the positive clones, T16, contained a cDNA sequence of 1934 nucleotides with a single open reading frame of 493 amino acids. A data base search revealed that T16 exhibited a strong sequence similarity to the human epidermal growth factor (EGF)-like protein, S(1-5). The region encoding amino acids 155-232 of DA41 was identified for the interaction with T16. Since DAN and S(1-5) proteins are known to suppress and stimulate DNA synthesis, respectively, it is possible that functional interaction of DAN with S(1-5) through DA41 might play an important role(s) in the regulation of cell growth.
最近，我们鉴定出一种能与候选肿瘤抑制蛋白DAN相结合的新蛋白（DA41）。在本研究中，我们利用酵母双杂交系统寻找与DA41相互作用的蛋白。使用缺乏DAN结合区域的截短形式的DA41（1-308）作为诱饵，筛选成年大鼠肺cDNA文库。其中一个阳性克隆T16包含一个1934个核苷酸的cDNA序列，带有一个493个氨基酸的单一开放阅读框。数据库搜索显示，T16与人类表皮生长因子（EGF）样蛋白S(1-5)表现出很强的序列相似性。已确定DA41编码氨基酸155-232的区域与T16相互作用。由于已知DAN和S(1-5)蛋白分别抑制和刺激DNA合成，因此DAN通过DA41与S(1-5)的功能相互作用可能在细胞生长调控中发挥重要作用。
