Solovyev A G, Zelenina D A, Savenkov E I, Grdzelishvili V Z, Maiss E, Casper R, Atabekov J G
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, russia.
Intervirology. 1997;40(1):1-6. doi: 10.1159/000150514.
The triple gene block (TGB) of barley stripe mosaic virus (BSMV), coding for viral movement proteins (MPs), was replaced by the single MP gene of red clover necrotic mosaic virus (RCNMV). Accumulation of the hybrid virus in barley plants (the selective host for BSMV) was reduced compared to BSMV. The hybrid virus induced small necrotic local lesions on Chenopodium amaranticolor leaves and did not infect Nicotiana clevelandii (the selective host for RCNMV). The hybrid virus accumulated in the inoculated leaves of Nicotiana benthamiana, but not in the upper noninoculated leaves. Thus the RCNMV MP gene substituted for the BSMV TGB in cell-to-cell movement, but not in systemic spread. Hybrid virus movement was efficient only in N. benthamiana, the common host for BSMV and RCNMV. These data point to the involvement of host-specific factors in the function of virus-coded transport determinants.
大麦条纹花叶病毒（BSMV）的三基因块（TGB）编码病毒运动蛋白（MP），被红三叶草坏死花叶病毒（RCNMV）的单个MP基因所取代。与BSMV相比，杂种病毒在大麦植株（BSMV的选择性寄主）中的积累减少。杂种病毒在苋色藜叶片上诱导产生小的坏死局部病斑，且不感染克利夫兰烟（RCNMV的选择性寄主）。杂种病毒在本氏烟草的接种叶片中积累，但在上部未接种叶片中不积累。因此，RCNMV的MP基因在细胞间移动中取代了BSMV的TGB，但在系统传播中并非如此。杂种病毒的移动仅在BSMV和RCNMV的共同寄主本氏烟草中有效。这些数据表明寄主特异性因子参与了病毒编码的运输决定因子的功能。
