Vaewhongs A A, Lommel S A
Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616, USA.
Virology. 1995 Oct 1;212(2):607-13. doi: 10.1006/viro.1995.1518.
The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species. The polycistronic RNA-1 encodes the viral RNA polymerase and capsid protein (CP) and the monocistronic RNA-2 encodes the 35 kDa cell-to-cell movement protein (MP). Nicotiana benthamiana plants transformed with the RCNMV MP gene were generated. When inoculated onto the MP transgenic plants, cell-to-cell movement of RNA-1 occurred at a rate similar to wild-type virus. However, long-distance (leaf-to-leaf) movement of RNA-1 was not observed. Neither CP nor virions were detected in the inoculated leaves of the MP transgenic plants. When RNA-1 was coinoculated with RNA-2 mutants, which do not express a functional MP, onto MP transgenic plants, CP and virions were readily detected and a systemic infection resulted. These results demonstrate that both RNA-1 and RNA-2 are necessary for the accumulation of both CP and virions. Furthermore, CP accumulation was found to be required for long-distance movement of RCNMV. Therefore, these data provide evidence that CP, in the form of virions, is necessary for the long-distance movement of RCNMV.
红三叶草坏死花叶 Dianthovirus（RCNMV）基因组分布在两个单链RNA分子上。多顺反子RNA-1编码病毒RNA聚合酶和衣壳蛋白（CP），单顺反子RNA-2编码35 kDa的细胞间运动蛋白（MP）。构建了用RCNMV MP基因转化的本氏烟草植株。当接种到MP转基因植株上时，RNA-1的细胞间运动速率与野生型病毒相似。然而，未观察到RNA-1的长距离（叶到叶）运动。在MP转基因植株的接种叶片中未检测到CP和病毒粒子。当RNA-1与不表达功能性MP的RNA-2突变体共同接种到MP转基因植株上时，很容易检测到CP和病毒粒子，并导致系统感染。这些结果表明，RNA-1和RNA-2对于CP和病毒粒子的积累都是必需的。此外，发现CP积累是RCNMV长距离运动所必需的。因此，这些数据提供了证据，表明病毒粒子形式的CP对于RCNMV的长距离运动是必需的。
