Ji D D, Arnot D E
Institute of Cell, Animal and Population Biology, Ashworth Laboratory, University of Edinburgh, UK.
Mol Biochem Parasitol. 1997 Sep;88(1-2):151-62. doi: 10.1016/s0166-6851(97)00089-3.
A Plasmodium falciparum homologue of one of the components of a chromatin-remodelling complex which controls binding of transcription factors to nucleosome core particles has been cloned and characterised. The gene encodes 1422 amino acids with an estimated molecular mass of 167 kDa. The protein, SNF2L, shares 60% amino acid identity in its conserved DNA-dependent ATPase domain with yeast transcription factors originally identified by characterising mating type switch mutants. It also contains sequences related to the so-called SWI3, ADA2, N-CoR and TFIIIB B" or SANT DNA binding domains which are characteristic of these transcriptional activation factors. The SNF2L gene has two short introns in the 3' region of the coding sequence of the gene and is transcribed into a single approximately 6.5 kb messenger RNA species which is present throughout the asexual stages of the cell cycle. Southern blotting and pulsed field gel electrophoresis experiments show that SNF2L is a single copy gene. located on P. falciparum chromosome 11.
已克隆并鉴定出一种与染色质重塑复合体的一个组分同源的恶性疟原虫基因，该复合体控制转录因子与核小体核心颗粒的结合。该基因编码1422个氨基酸，估计分子量为167 kDa。蛋白质SNF2L在其保守的依赖DNA的ATP酶结构域中与最初通过鉴定交配型转换突变体而确定的酵母转录因子具有60%的氨基酸同一性。它还包含与所谓的SWI3、ADA2、N-CoR和TFIIIB B" 或SANT DNA结合结构域相关的序列，这些结构域是这些转录激活因子的特征。SNF2L基因在基因编码序列的3' 区域有两个短内含子，并转录成单一的约6.5 kb信使RNA，在细胞周期的无性阶段均有表达。Southern印迹和脉冲场凝胶电泳实验表明，SNF2L是一个单拷贝基因，位于恶性疟原虫11号染色体上。
