Wang Y H, Allardyce R A, Rogers G R, Gillespie W J, Doyle J S
Department of Surgery, Wellington School of Medicine, New Zealand.
Chin Med J (Engl). 1996 Sep;109(9):711-9.
To investigate the relationships between degradation of bone and activated blood polymorphonuclear leukocytes (PMNL) and mononuclear leukocytes (ML) as well as their soluble products in vitro.
A neonatal mouse calvarial bone model was used to assess the activity of degradation (by measuring the amount of 45Ca release) by normal human blood leukocytes, separated PMNL and ML following 24-hour incubation. The effects of conditioned culture medium obtained from Staphylococcus aureus-stimulated ML on PMNL-mediated calvarial bone loss were also studied.
It was demonstrated that isolated human PMNL rapidly degraded bone in a dose and time dependent manner. The PMNL-mediated bone degradation was enhanced by conditioned medium obtained from Staphylococcus aureus-stimulated ML.
These findings implicate PMNL as major contributors to early bone loss in infectious diseases such as acute haematogenous osteomyelitis.
研究体外骨降解与活化的血液多形核白细胞（PMNL）和单核白细胞（ML）及其可溶性产物之间的关系。
采用新生小鼠颅骨模型，通过测量45Ca释放量来评估正常人血液白细胞、分离的PMNL和ML在孵育24小时后的降解活性。还研究了金黄色葡萄球菌刺激的ML获得的条件培养基对PMNL介导的颅骨骨丢失的影响。
结果表明，分离的人PMNL以剂量和时间依赖性方式快速降解骨。金黄色葡萄球菌刺激的ML获得的条件培养基增强了PMNL介导的骨降解。
这些发现表明PMNL是急性血源性骨髓炎等传染病早期骨丢失的主要促成因素。
