Yang B, Shen Y, Ma L
Division of Nephrology, First Hospital, Beijing Medical University.
Zhonghua Yi Xue Za Zhi. 1996 Jun;76(6):416-9.
To study the regulative effects of rhIL-1 beta or rhTNF-alpha on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on human mesangial cells (HMC).
At the 4th, 8th, 16th and 32nd hours after stimulation by rhIL-1 beta (25 ng/ml) or rhTNF alpha (100 ng/ml), the mRNA expression of ICAM-1 and VCAM-1 on HMC was determined with Northern blot, and their protein expression was also tested with cell ELISA analysis.
mRNA and protein of both ICAM-1 and VCAM-1 were only basically expressed on HMC at very low levels in the control groups without stimulation. After stimulation by rhIL-1 beta or rhTNF alpha, however, their expression was markedly upregulated. The maximal mRNA expression level of ICAM-1 stimulated by rhTNF alpha was present at the 8th hour, but in the remaining groups the maximal mRNA expression levels of ICAM-1 or VCAM-1 were all present at the 4th hour. Compared with control groups, ICAM-1 protein expression was significantly increased from the 4th hour after rhIL-1 beta stimulation (P < 0.05), but in the remaining groups ICAM-1 or VCAM-1 protein expression was significantly raised from the 8th hour after stimulation (P < 0.001).
These results suggest that upregulated expression of ICAM-1 and VCAM-1 by inflammatory cytokines IL-1 beta and TNF alpha may play a pathogenic role in glomerulonephritis.
研究重组人白细胞介素-1β（rhIL-1β）或重组人肿瘤坏死因子-α（rhTNF-α）对人系膜细胞（HMC）细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）表达的调节作用。
用rhIL-1β（25 ng/ml）或rhTNF-α（100 ng/ml）刺激后第4、8、16和32小时，采用Northern印迹法检测HMC上ICAM-1和VCAM-1的mRNA表达，并用细胞酶联免疫吸附分析检测其蛋白表达。
在无刺激的对照组中，ICAM-1和VCAM-1的mRNA和蛋白仅在HMC上以极低水平基本表达。然而，经rhIL-1β或rhTNF-α刺激后，它们的表达明显上调。rhTNF-α刺激后ICAM-1的最大mRNA表达水平出现在第8小时，但在其余组中，ICAM-1或VCAM-1的最大mRNA表达水平均出现在第4小时。与对照组相比，rhIL-1β刺激后第4小时ICAM-1蛋白表达显著增加（P<0.05），但在其余组中，刺激后第8小时ICAM-1或VCAM-1蛋白表达显著升高（P<0.001）。
这些结果表明，炎性细胞因子IL-1β和TNF-α上调ICAM-1和VCAM-1的表达可能在肾小球肾炎中起致病作用。
