Wu K, Rooney M F, Ferl R J
Horticultural Sciences Department, University of Florida, Gainesville 32611, USA.
Plant Physiol. 1997 Aug;114(4):1421-31. doi: 10.1104/pp.114.4.1421.
The 14-3-3 proteins are ubiquitous eukaryotic proteins and are encoded by a gene family in many species. We examined the 14-3-3 gene family in Arabidopsis thaliana and found that it contains 10 members. Four new cDNAs, GF14 epsilon, GF14 kappa, GF14 mu, and GF14 nu, and two new genomic clones of GF14 phi and GF14 nu were isolated and characterized. Together with the six previously described 14-3-3 isoforms in Arabidopsis, they constitute a complete family of 10 distinct 14-3-3 proteins of 248 to 268 amino acids. Phylogenetic analysis revealed the presence of two ancient, distinct 14-3-3 gene classes in Arabidopsis and other plants. The epsilon forms diverged early from the other plant isoforms, and plant 14-3-3 genes displayed a different evolutionary course from that of mammals.
14-3-3蛋白是普遍存在的真核生物蛋白，在许多物种中由一个基因家族编码。我们研究了拟南芥中的14-3-3基因家族，发现它包含10个成员。分离并鉴定了四个新的cDNA，即GF14ε、GF14κ、GF14μ和GF14ν，以及两个新的GF14φ和GF14ν基因组克隆。它们与拟南芥中先前描述的六种14-3-3同工型一起，构成了一个由10种不同的14-3-3蛋白组成的完整家族，这些蛋白由248至268个氨基酸组成。系统发育分析表明，拟南芥和其他植物中存在两个古老的、不同的14-3-3基因类群。ε型与其他植物同工型早期分化，植物14-3-3基因显示出与哺乳动物不同的进化历程。
