Chang J P, Kiehl D E, Kennington A
Lilly Research Laboratories, Eli Lilly and Co., Greenfield, IN 46140, USA.
Rapid Commun Mass Spectrom. 1997;11(12):1266-70. doi: 10.1002/(SICI)1097-0231(199708)11:12<1266::AID-RCM977>3.0.CO;2-3.
Reversed phase high-performance liquid chromatography (RPHPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) was used to separate and characterize the peptides resulting from the tryptic cleavage of somidobove, a recombinant bovine growth hormone. The tryptic digestion of somidobove was carried out at room temperature for 15 hours. The tryptic peptides were separated on a Zorbax SB300 C8 column with trifluoroacetic acid--acetonitrile gradient elution and characterized by LC/ESI-MS. Resulting single and combined peptide fragments were characterized by comparing the observed molecular mass with the calculated mass for these fragments.
反相高效液相色谱（RPHPLC）与电喷雾电离质谱（ESI-MS）联用，用于分离和鉴定重组牛生长激素——生长素释放肽经胰蛋白酶酶解产生的肽段。生长素释放肽的胰蛋白酶消化在室温下进行15小时。胰蛋白酶肽段在Zorbax SB300 C8柱上采用三氟乙酸-乙腈梯度洗脱进行分离，并用LC/ESI-MS进行鉴定。通过将观察到的分子量与这些片段的计算分子量进行比较，对产生的单个和组合肽片段进行鉴定。
