使用阴离子交换高效液相色谱法分析心脏组织中的肌醇磷酸酯。

Analysis of inositol phosphates in heart tissue using anion-exchange high-performance liquid chromatography.

作者信息

Woodcock E A

机构信息

Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran, Victoria, Australia.

出版信息

Mol Cell Biochem. 1997 Jul;172(1-2):121-7.

PMID:9278239

Abstract

The pathways of release and metabolism of inositol phosphates in intact heart tissue are different from those observed in isolated cardiomyocytes in culture. Thus, it is essential that methods are available for the quantitation of inositol phosphates in intact tissue preparations. This manuscript describes methods which allow the quantitation of inositol phosphates in different heart preparations including isolated atria and intact perfused heart. The availability of such methods should facilitate study of the role of inositol phosphates in cardiac control mechanisms under physiological and pathological conditions.

摘要

完整心脏组织中肌醇磷酸的释放和代谢途径与培养的分离心肌细胞中观察到的不同。因此，必须有可用于定量完整组织制剂中肌醇磷酸的方法。本手稿描述了可对不同心脏制剂（包括分离的心房和完整的灌注心脏）中的肌醇磷酸进行定量的方法。这些方法的可用性应有助于研究肌醇磷酸在生理和病理条件下心脏控制机制中的作用。