类Elav蛋白与富含AU的元件以及mRNA的聚腺苷酸尾相结合。
The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA.
作者信息
Ma W J, Chung S, Furneaux H
机构信息
Program in Molecular Pharmacology and Therapeutics, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021 USA.
出版信息
Nucleic Acids Res. 1997 Sep 15;25(18):3564-9. doi: 10.1093/nar/25.18.3564.
Abstract
The Elav-like proteins are specific mRNA binding proteins which are required for cellular differentiation. They contain three characteristic RNP2/RNP1-type RNA binding motifs. Previously we have shown that the first and second RNA binding domains bind to AU-rich elements in the 3'-UTR of mRNA. In this paper we show that the Elav-like proteins exhibit poly(A) binding activity. This activity is distinct from poly(A) binding activities that have been previously described. The Elav-like proteins specifically bind to long chain poly(A) tails. We have shown that the third RNA binding domain encompasses this poly(A) binding activity. Using poly(A)-Sepharose beads in a 'sandwich' assay we have shown that the Elav-like proteins can bind simultaneously to the AU-rich element and to the poly(A) tail.
摘要
类Elav蛋白是细胞分化所必需的特异性mRNA结合蛋白。它们包含三个特征性的RNP2/RNP1型RNA结合基序。此前我们已表明，第一个和第二个RNA结合结构域与mRNA 3'-UTR中的富含AU元件结合。在本文中，我们表明类Elav蛋白具有聚腺苷酸（poly(A)）结合活性。这种活性不同于先前描述的聚腺苷酸结合活性。类Elav蛋白特异性结合长链聚腺苷酸尾巴。我们已表明，第三个RNA结合结构域包含这种聚腺苷酸结合活性。在“三明治”试验中使用聚腺苷酸琼脂糖珠，我们已表明类Elav蛋白可同时结合富含AU元件和聚腺苷酸尾巴。
相似文献
1
The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA.类Elav蛋白与富含AU的元件以及mRNA的聚腺苷酸尾相结合。
Nucleic Acids Res. 1997 Sep 15;25(18):3564-9. doi: 10.1093/nar/25.18.3564.
2
ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.ELAV蛋白在一种新型的体外mRNA去腺苷酸化/降解系统中稳定去腺苷酸化中间体。
Genes Dev. 1999 Jan 15;13(2):188-201. doi: 10.1101/gad.13.2.188.
3
ELAV multimerizes on conserved AU4-6 motifs important for ewg splicing regulation.ELAV在对ewg剪接调控很重要的保守AU4-6基序上多聚化。
Mol Cell Biol. 2005 Sep;25(17):7580-91. doi: 10.1128/MCB.25.17.7580-7591.2005.
4
Mammalian homologs of Drosophila ELAV localized to a neuronal subset can bind in vitro to the 3' UTR of mRNA encoding the Id transcriptional repressor.果蝇ELAV的哺乳动物同源物定位于一个神经元亚群，在体外可与编码Id转录抑制因子的mRNA的3'非翻译区结合。
J Neurosci. 1994 Apr;14(4):1943-52. doi: 10.1523/JNEUROSCI.14-04-01943.1994.
5
The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis.非洲爪蟾的ELAV蛋白ElrB在卵子发生过程中抑制Vg1 mRNA的翻译。
Mol Cell Biol. 2005 Oct;25(20):9028-39. doi: 10.1128/MCB.25.20.9028-9039.2005.
6
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.HuR的克隆与鉴定，一种广泛表达的类Elav蛋白。
J Biol Chem. 1996 Apr 5;271(14):8144-51. doi: 10.1074/jbc.271.14.8144.
7
Stability of casein mRNA is ensured by structural interactions between the 3'-untranslated region and poly(A) tail via the HuR and poly(A)-binding protein complex.酪蛋白信使核糖核酸的稳定性是通过3'非翻译区与多聚腺苷酸尾之间经由HuR和多聚腺苷酸结合蛋白复合体的结构相互作用来确保的。
Biochim Biophys Acta. 2006 Mar-Apr;1759(3-4):132-40. doi: 10.1016/j.bbaexp.2006.04.004. Epub 2006 Apr 20.
8
ELAV tumor antigen, Hel-N1, increases translation of neurofilament M mRNA and induces formation of neurites in human teratocarcinoma cells.ELAV肿瘤抗原Hel-N1可增加神经丝蛋白M mRNA的翻译，并诱导人畸胎瘤细胞中神经突的形成。
Genes Dev. 1999 Feb 15;13(4):449-61. doi: 10.1101/gad.13.4.449.
9
Binding of neuronal ELAV-like proteins to the uridine-rich sequence in the 3'-untranslated region of tumor necrosis factor-alpha messenger RNA.神经元ELAV样蛋白与肿瘤坏死因子-α信使核糖核酸3'-非翻译区富含尿苷的序列的结合。
FEBS Lett. 1999 Mar 5;446(1):157-62. doi: 10.1016/s0014-5793(99)00206-9.
10
RNA-binding analyses of HuC and HuD with the VEGF and c-myc 3'-untranslated regions using a novel ELISA-based assay.使用基于酶联免疫吸附测定（ELISA）的新型检测方法，对HuC和HuD与血管内皮生长因子（VEGF）和c-myc 3'非翻译区进行RNA结合分析。
Nucleic Acids Res. 2000 Apr 1;28(7):E20. doi: 10.1093/nar/28.7.e20.
引用本文的文献
1
Multifaceted Human Antigen R (HuR): A Key Player in Liver Metabolism and MASLD.多面手人类抗原R（HuR）：肝脏代谢和代谢相关脂肪性肝病中的关键角色
Livers. 2025 Sep;5(3). doi: 10.3390/livers5030033. Epub 2025 Jul 21.
2
Mechanism of expression regulation of head-to-head overlapping protein-coding genes INO80E and HIRIP3.头对头重叠蛋白编码基因INO80E和HIRIP3的表达调控机制
Commun Biol. 2025 Mar 8;8(1):391. doi: 10.1038/s42003-025-07815-4.
3
ELAV/Hu RNA-binding protein family: key regulators in neurological disorders, cancer, and other diseases.ELAV/Hu RNA结合蛋白家族：神经疾病、癌症及其他疾病中的关键调节因子。
RNA Biol. 2025 Dec;22(1):1-11. doi: 10.1080/15476286.2025.2471133. Epub 2025 Mar 20.
4
Structural, biological, and biomedical implications of mRNA interactions with the master regulator HuR.信使核糖核酸（mRNA）与主要调控因子HuR相互作用的结构、生物学及生物医学意义
NAR Mol Med. 2025 Feb 3;2(1):ugaf002. doi: 10.1093/narmme/ugaf002. eCollection 2025 Jan.
5
Role of Post-Transcriptional Regulation in Learning and Memory in Mammals.哺乳动物学习和记忆中的转录后调控作用。
Genes (Basel). 2024 Mar 5;15(3):337. doi: 10.3390/genes15030337.
6
Post-transcriptional regulation of BIRC5/survivin expression and induction of apoptosis in breast cancer cells by tristetraprolin.Tristetraprolin 对乳腺癌细胞 BIRC5/survivin 表达的转录后调控及诱导细胞凋亡
RNA Biol. 2024 Jan;21(1):1-15. doi: 10.1080/15476286.2023.2286101. Epub 2023 Dec 18.
7
The molecular genetics of nELAVL in brain development and disease.ELAVL 蛋白在脑发育和疾病中的分子遗传学。
Eur J Hum Genet. 2023 Nov;31(11):1209-1217. doi: 10.1038/s41431-023-01456-z. Epub 2023 Sep 12.
8
Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids.整合转录组和蛋白质组分析揭示了人类脑类器官中核糖体基因的转录后调控。
Elife. 2023 Mar 29;12:e85135. doi: 10.7554/eLife.85135.
9
The Prognostic Role of HuR Varies Between Different Subtypes of Breast Cancer Patients: Data Mining and Retrospective Analysis.HuR的预后作用在不同亚型乳腺癌患者中存在差异：数据挖掘与回顾性分析
Breast Cancer (Dove Med Press). 2023 Feb 11;15:135-146. doi: 10.2147/BCTT.S395984. eCollection 2023.
10
The versatile role of HuR in Glioblastoma and its potential as a therapeutic target for a multi-pronged attack.HuR 在胶质母细胞瘤中的多功能作用及其作为多管齐下治疗靶点的潜力。
Adv Drug Deliv Rev. 2022 Feb;181:114082. doi: 10.1016/j.addr.2021.114082. Epub 2021 Dec 16.
本文引用的文献
1
A New Phase for The AJP.《美国生理学杂志》的新阶段。
Am J Pathol. 1992 Jul;141(1):1.
2
Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.鉴定HuR为一种与AUUUA介导的mRNA降解相关的蛋白质。
EMBO J. 1997 Apr 15;16(8):2130-9. doi: 10.1093/emboj/16.8.2130.
3
The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mRNA.Elav样蛋白与GAP-43 mRNA 3'非翻译区的一个保守调控元件结合。
J Biol Chem. 1997 Mar 7;272(10):6593-8. doi: 10.1074/jbc.272.10.6593.
4
Two different RNA binding activities for the AU-rich element and the poly(A) sequence of the mouse neuronal protein mHuC.针对小鼠神经元蛋白mHuC富含AU元件和聚腺苷酸序列的两种不同RNA结合活性。
Nucleic Acids Res. 1996 Dec 15;24(24):4895-901. doi: 10.1093/nar/24.24.4895.
5
Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.RNA结合蛋白Hel-N1的异位表达增加了3T3-L1脂肪细胞中葡萄糖转运蛋白（GLUT1）的表达。
Mol Cell Biol. 1997 Feb;17(2):954-62. doi: 10.1128/MCB.17.2.954.
6
Purification and properties of HuD, a neuronal RNA-binding protein.神经元RNA结合蛋白HuD的纯化与特性
J Biol Chem. 1996 May 10;271(19):11518-24. doi: 10.1074/jbc.271.19.11518.
7
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.HuR的克隆与鉴定，一种广泛表达的类Elav蛋白。
J Biol Chem. 1996 Apr 5;271(14):8144-51. doi: 10.1074/jbc.271.14.8144.
8
Hu protein as an early marker of neuronal phenotypic differentiation by subependymal zone cells of the adult songbird forebrain.胡蛋白作为成年鸣禽前脑室管膜下区细胞神经元表型分化的早期标志物。
J Neurobiol. 1995 Sep;28(1):82-101. doi: 10.1002/neu.480280108.
9
Two distinct temperature-sensitive alleles at the elav locus of Drosophila are suppressed nonsense mutations of the same tryptophan codon.果蝇elav基因座上的两个不同的温度敏感等位基因是同一色氨酸密码子的抑制性无义突变。
Genetics. 1995 Nov;141(3):1101-11. doi: 10.1093/genetics/141.3.1101.
10
Tissue-specific expression of the gene encoding a mouse RNA binding protein homologous to human HuD antigen.编码与人类HuD抗原同源的小鼠RNA结合蛋白的基因的组织特异性表达。
DNA Res. 1994;1(4):175-80. doi: 10.1093/dnares/1.4.175.
Zotero 插件