体内和体外的蛋白质磷酸化与牛磺酸生物合成
Protein phosphorylation and taurine biosynthesis in vivo and in vitro.
作者信息
Tang X W, Hsu C C, Schloss J V, Faiman M D, Wu E, Yang C Y, Wu J Y
机构信息
Department of Physiology and Cell Biology, University of Kansas, Lawrence, Kansas 66045-2106, USA.
出版信息
J Neurosci. 1997 Sep 15;17(18):6947-51. doi: 10.1523/JNEUROSCI.17-18-06947.1997.
Abstract
Taurine is known to be involved in many important physiological functions. Here we report that both in vivo and in vitro the taurine-synthesizing enzyme in the brain, namely cysteine sulfinic acid decarboxylase (CSAD), is activated when phosphorylated and inhibited when dephosphorylated. Furthermore, protein kinase C and protein phosphatase 2C have been identified as the enzymes responsible for phosphorylation and dephosphorylation of CSAD, respectively. In addition, the effect of neuronal depolarization on CSAD activity and 32P incorporation into CSAD in neuronal cultures is also included. A model to link neuronal excitation and CSAD activation by a Ca2+-dependent protein kinase is proposed.
摘要
已知牛磺酸参与许多重要的生理功能。在此我们报告，在体内和体外，大脑中的牛磺酸合成酶，即半胱氨酸亚磺酸脱羧酶（CSAD），在磷酸化时被激活，在去磷酸化时被抑制。此外，蛋白激酶C和蛋白磷酸酶2C已分别被确定为负责CSAD磷酸化和去磷酸化的酶。另外，还包括神经元去极化对神经元培养物中CSAD活性和32P掺入CSAD的影响。提出了一个通过钙依赖蛋白激酶将神经元兴奋与CSAD激活联系起来的模型。
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