通过聚合酶链反应（PCR）扩增因子VIII基因的Bcl I区域。

Amplification of Bcl I region of the factor VIII gene by PCR.

作者信息

Ishak R, Khim L C

机构信息

Hematology Division, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.

出版信息

Southeast Asian J Trop Med Public Health. 1996 Jun;27(2):364-6.

PMID:9280004

Abstract

A study was initiated to amplify by polymerase chain reaction (PCR), a short factor VIII gene fragment containing the Bcl I restriction site from hemophilia patients using published primer sequences. Preliminary findings indicated that the resulting fragment is 142 bp long. This fragment, when digested with Bcl I restriction enzyme produced two fragments, 99 bp and 43 bp in length. Polymorphism in the Bcl I region can be used to detect carrier state in the family members of the hemophiliacs.

摘要

开展了一项研究，使用已发表的引物序列，通过聚合酶链反应（PCR）扩增血友病患者中包含Bcl I限制性酶切位点的短因子VIII基因片段。初步研究结果表明，所得片段长度为142 bp。该片段用Bcl I限制性内切酶消化后产生两个片段，长度分别为99 bp和43 bp。Bcl I区域的多态性可用于检测血友病患者家庭成员的携带者状态。