Norman J E, Gray M W
Program in Evolutionary Biology, Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia.
FEBS Lett. 1997 Aug 18;413(2):333-8. doi: 10.1016/s0014-5793(97)00938-1.
To date, no genes have been characterized from dinoflagellate mitochondrial DNA. Here we present the complete sequence of the gene (cox1) encoding subunit 1 of cytochrome c oxidase in the dinoflagellate, Crypthecodinium cohnii. Analysis of nucleotide and deduced amino acid sequences predicts a protein of 523 amino acids that is translated using universal initiation, stop and tryptophan codons. COX1 amino acid identity and phylogenetic tree analyses strongly support a close evolutionary relationship between dinoflagellates and apicomplexans; however, inclusion of the ciliates in this clade is less well supported, a result likely due to the highly derived nature of ciliate COX1 sequences.
迄今为止,尚未对甲藻线粒体DNA中的基因进行表征。在此,我们展示了甲藻隐甲藻(Crypthecodinium cohnii)中编码细胞色素c氧化酶亚基1的基因(cox1)的完整序列。对核苷酸和推导的氨基酸序列分析预测,该蛋白由523个氨基酸组成,其翻译使用通用起始密码子、终止密码子和色氨酸密码子。COX1氨基酸同一性和系统发育树分析有力地支持了甲藻和顶复门生物之间存在密切的进化关系;然而,将纤毛虫纳入该进化枝的支持度较低,这可能是由于纤毛虫COX1序列具有高度衍生的性质。