Ferrer O, Real F, Molina J M, Acosta B, Munoz M C, Leon L
Departamento de Patología Animal, Facultad Veterinaria de Las Palmas de G.C., Gran Canaria, Spain.
Comp Immunol Microbiol Infect Dis. 1997 Jun;20(3):253-60. doi: 10.1016/s0147-9571(96)00034-3.
Radial immunodiffusion was used to determine immunoglobulin (Ig)-G concentrations in 16 mammary secretions from uninfected udder halves and in 10-14 secretions from halves subclinically infected with coagulase-negative staphylococci in goats throughout lactation. IgG concentrations in samples from uninfected halves decreased rapidly during the first week after parturition (P < 0.001 ), henceforth falling slowly up to 30 d post-partum (P < 0.001). From 30 d post-partum to 180 d of lactation, IgG concentration showed a continuous decline to the end of the experiment but these changes were not statistically significant until 150 d after kidding (P < 0.01). IgG concentrations in lacteal secretions of halves harbouring coagulase-negative staphylococci showed a similar pattern, from the first month of lactation, to that observed in healthy udder halves, but concentrations were always lower. These differences were significant from 2 months after parturition (P < 0.001). IgG concentrations were compared with somatic cell counts obtained using an automatic counter (Fossomatic).
采用放射免疫扩散法测定了山羊整个泌乳期未感染乳腺半侧的16份乳汁分泌物以及亚临床感染凝固酶阴性葡萄球菌的乳腺半侧的10 - 14份分泌物中的免疫球蛋白（Ig）-G浓度。未感染半侧样本中的IgG浓度在分娩后的第一周迅速下降（P < 0.001），此后直至产后30天缓慢下降（P < 0.001）。从产后30天到泌乳180天，IgG浓度持续下降直至实验结束，但这些变化在产羔后150天之前无统计学意义（P < 0.01）。携带凝固酶阴性葡萄球菌的乳腺半侧的乳汁分泌物中的IgG浓度从泌乳第一个月起呈现出与健康乳腺半侧相似的模式，但浓度始终较低。这些差异在分娩后2个月时显著（P < 0.001）。将IgG浓度与使用自动计数器（Fossomatic）获得的体细胞计数进行了比较。
