Lauer U, Bürgelt E, Squire Z, Messmer K, Hofschneider P H, Gregor M, Delius M
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Gene Ther. 1997 Jul;4(7):710-5. doi: 10.1038/sj.gt.3300462.
Uptake of naked functional DNA into mammalian cells can be achieved by a number of physical methods. However, for most of these techniques possibilities for therapeutic in vivo applications--especially to solid organs--are often limited. In this report, we describe shock wave permeabilization as a new physical gene transfer method, which can be easily applied, provides great flexibility in the size and sequence of the DNA molecules to be delivered, and which should exhibit an advantageous security profile in vivo. Upon exposure to lithotripter-generated shock waves eukaryotic cells display a temporary increase in membrane permeability. This effect was shown to be caused by cavitation resulting in the transient generation of cell pores which allows the direct transfer of naked plasmid DNA. Shockwave transfection of a variety of cell lines was demonstrated. Since shock waves can be well focused within particular body regions, future applications of extracorporally generated shock waves to tissues simultaneously perfused with DNA solutions might open up the possibility of achieving a regionally enhanced in vivo gene transfer.
通过多种物理方法可实现将裸露的功能性DNA导入哺乳动物细胞。然而，对于这些技术中的大多数而言，其体内治疗应用的可能性——尤其是对实体器官的应用——往往受到限制。在本报告中，我们描述了冲击波通透化作为一种新的物理基因转移方法，它易于应用，在待递送的DNA分子的大小和序列方面具有很大的灵活性，并且在体内应具有有利的安全性。暴露于碎石机产生的冲击波后，真核细胞的膜通透性会暂时增加。这种效应被证明是由空化作用引起的，空化作用导致细胞孔隙的短暂形成，从而允许裸露的质粒DNA直接转移。已证明对多种细胞系进行冲击波转染。由于冲击波可以很好地聚焦在特定的身体区域，因此将体外产生的冲击波应用于同时灌注DNA溶液的组织的未来应用可能会开辟实现体内区域增强基因转移的可能性。
