Mitskavich M T, Rimell F L, Shapiro A M
Department of Pediatric Otolaryngology, Children's Hospital of Pittsburgh, PA, USA.
Am J Otolaryngol. 1997 Sep-Oct;18(5):315-9. doi: 10.1016/s0196-0709(97)90025-9.
The development of a reliable animal model of laryngeal or tracheal stenosis has been limited by lack of reproducibility, high morbidity and mortality, and cumbersome technique. The small size of previously proposed models has limited the development of innovative reconstructive techniques.
Eleven of 15 post-weanling commercial piglets underwent injury by a sharpened metal rod to the anterior half of the subglottic region at repeated intervals under endoscopic guidance. Four of 15 animals did not undergo injury and served as control for normal subglottic growth over time. Each airway was sized by endotracheal tube (ETT) passage and endoscopic photography over time, and the degree of total stenosis was estimated in comparison to the control animals.
The first two animals underwent daily injury and expired from copious granulation tissue. Eight additional animals developed symptomatic subglottic stenosis manifested by audible stridor and intercostal retractions while undergoing injury every third day. Photographs documented the size of the stenosis to be greater than 50% (40% by ETT sizing) with a mean of three injuries in a mean time of 18 days.
The porcine model shows a high degree of tolerance with minimal morbidity and consistent reliable results in an animal model of airway stenosis that is suitable for further innovative studies.
由于缺乏可重复性、高发病率和死亡率以及技术繁琐，可靠的喉或气管狭窄动物模型的开发受到限制。先前提出的模型尺寸较小，限制了创新重建技术的发展。
15只断奶后的商用仔猪中有11只在反复间隔的时间内经内镜引导，用尖锐的金属棒损伤声门下区域的前半部分。15只动物中有4只未接受损伤，作为声门下随时间正常生长的对照。随着时间的推移，通过气管内插管（ETT）通过和内镜摄影对每个气道进行测量，并与对照动物比较估计总狭窄程度。
前两只动物每天接受损伤，因大量肉芽组织而死亡。另外8只动物在每三天接受一次损伤时出现有症状的声门下狭窄，表现为可闻及的喘鸣和肋间凹陷。照片记录狭窄尺寸大于50%（通过ETT测量为40%），平均在18天内平均接受三次损伤。
在气道狭窄的动物模型中，猪模型显示出高度耐受性，发病率极低且结果一致可靠，适用于进一步的创新研究。
