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A nonsubstituted primary hydroxyl group in position 6' of free lipid A is required for binding of lipid A monoclonal antibodies.游离脂质A的6'位上的非取代伯羟基是脂质A单克隆抗体结合所必需的。
Infect Immun. 1997 Sep;65(9):3961-5. doi: 10.1128/iai.65.9.3961-3965.1997.
2
An artificial glycoconjugate containing the bisphosphorylated glucosamine disaccharide backbone of lipid A binds lipid A monoclonal antibodies.一种含有脂多糖双磷酸化葡糖胺二糖主链的人工糖缀合物可结合脂多糖单克隆抗体。
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Mapping of the binding specificity for five monoclonal antibodies recognizing 3-deoxy-D-manno-octulosonic acid in bacterial lipopolysaccharides.识别细菌脂多糖中3-脱氧-D-甘露糖辛酮酸的五种单克隆抗体的结合特异性图谱
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4
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6
Determination of the epitope specificity of monoclonal antibodies against the inner core region of bacterial lipopolysaccharides by use of 3-deoxy-D-manno-octulosonate-containing synthetic antigens.利用含3-脱氧-D-甘露辛酮糖酸的合成抗原测定抗细菌脂多糖内核区域单克隆抗体的表位特异性
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Use of synthetic antigens to determine the epitope specificities of monoclonal antibodies against the 3-deoxy-D-manno-octulosonate region of bacterial lipopolysaccharide.使用合成抗原确定针对细菌脂多糖3-脱氧-D-甘露糖辛酮酸区域的单克隆抗体的表位特异性。
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Specificity of rabbit antisera against the rough lipopolysaccharide of Salmonella minnesota R4 (chemotype Rd2P-).兔抗明尼苏达沙门氏菌R4粗糙脂多糖（化学型Rd2P-）抗血清的特异性
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Structural Basis for Antibody Recognition of Lipid A: INSIGHTS TO POLYSPECIFICITY TOWARD SINGLE-STRANDED DNA.脂多糖A抗体识别的结构基础：对单链DNA多特异性的见解
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Glucosamine found as a substituent of both phosphate groups in Bordetella lipid A backbones: role of a BvgAS-activated ArnT ortholog.在博德特氏菌脂多糖A骨架的两个磷酸基团中发现的氨基葡萄糖：一种BvgAS激活的ArnT直系同源物的作用。
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Endotoxin-based molecular strategies for the prevention and treatment of gram-negative sepsis and septic shock.基于内毒素的预防和治疗革兰氏阴性菌败血症及感染性休克的分子策略。
Curr Top Microbiol Immunol. 1996;216:275-97. doi: 10.1007/978-3-642-80186-0_13.
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Bacterial endotoxin: Chemical constitution, biological recognition, host response, and immunological detoxification.细菌内毒素：化学组成、生物识别、宿主反应及免疫解毒
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Characterization of specific binding of a human immunoglobulin M monoclonal antibody to lipopolysaccharide and its lipid A domain.人免疫球蛋白M单克隆抗体与脂多糖及其脂质A结构域的特异性结合特性
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An artificial glycoconjugate containing the bisphosphorylated glucosamine disaccharide backbone of lipid A binds lipid A monoclonal antibodies.一种含有脂多糖双磷酸化葡糖胺二糖主链的人工糖缀合物可结合脂多糖单克隆抗体。
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Cross-reactivity of monoclonal antibodies and sera directed against lipid A and lipopolysaccharides.针对脂质A和脂多糖的单克隆抗体及血清的交叉反应性。
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Molecular structure of lipid A, the endotoxic center of bacterial lipopolysaccharides.脂质A的分子结构，细菌脂多糖的内毒素中心。
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游离脂质A的6'位上的非取代伯羟基是脂质A单克隆抗体结合所必需的。

A nonsubstituted primary hydroxyl group in position 6' of free lipid A is required for binding of lipid A monoclonal antibodies.

作者信息

Brade L, Engel R, Christ W J, Rietschel E T

机构信息

Department of Immunochemistry and Biochemical Microbiology, Center for Medicine and Biosciences, Research Center Borstel, Germany.

出版信息

Infect Immun. 1997 Sep;65(9):3961-5. doi: 10.1128/iai.65.9.3961-3965.1997.

DOI:10.1128/iai.65.9.3961-3965.1997
PMID:9284181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC175568/
Abstract

Lipid A monoclonal antibodies, which require for binding the presence of the bisphosphorylated D-glucosamine disaccharide lipid A backbone, were tested against synthetic lipid A precursor Ia and compound B 1047 by enzyme immunoassay. The last-named compound is a precursor Ia analog with a methoxy instead of a hydroxy group at C6' and was chosen to determine why these antibodies failed to recognize the bound lipid A present in lipopolysaccharide (LPS). Whereas all antibodies tested bound to precursor Ia, none of them bound to compound B 1047 or Escherichia coli Re LPS to a significant extent. Compared to the natural substituent at C6', i.e., 3-deoxy-D-manno-octulosonic acid (Kdo), the methoxy group is neither bulky nor charged. Thus, the data suggest that it is not hindrance by Kdo but rather the generation of a neoantigen that endows lipid A with immunoreactivity upon liberation from LPS by acid hydrolysis.

摘要

脂质A单克隆抗体需要双磷酸化的D-葡糖胺二糖脂质A主链才能结合，通过酶免疫测定法对合成脂质A前体Ia和化合物B 1047进行了测试。后一种化合物是前体Ia类似物，在C6'处有一个甲氧基而非羟基，选择它是为了确定为什么这些抗体无法识别脂多糖（LPS）中结合的脂质A。尽管所有测试的抗体都与前体Ia结合，但它们都没有与化合物B 1047或大肠杆菌Re LPS发生显著结合。与C6'处的天然取代基即3-脱氧-D-甘露糖辛酸（Kdo）相比，甲氧基既不庞大也不带电荷。因此，数据表明，赋予脂质A免疫反应性的不是Kdo的阻碍，而是在通过酸水解从LPS释放后产生了一种新抗原。