从公猪晚期精子细胞核中分离出具有三个锌指基序的完整过渡蛋白2。

Isolation of intact transition protein 2 with three zinc finger motifs from boar late spermatid nuclei.

作者信息

Akama K, Sato H, Oguma K, Nakano M

机构信息

Department of Chemistry, Faculty of Science, Chiba University, Japan.

出版信息

Biochem Mol Biol Int. 1997 Aug;42(5):865-72. doi: 10.1080/15216549700203301.

DOI:10.1080/15216549700203301

PMID:9285053

Abstract

Boar intact transition protein 2, TP2, was isolated from the late spermatid nuclei by chromatography on Fractogel EMD SO3- 650 (M), and HPLCs on Nucleosil 300 7C18, Diol-120 and Chemcosorb 3C18H. CD spectroscopy study showed that TP2 underwent a small but significant zinc dependent secondary structural change. TP2, having three potential zinc finger motifs, was shown to be contain 3 atoms of zinc bound per molecule of the protein by atomic absorption spectroscopy. These results together with the amino acid sequence of TP2 suggest that TP2 is a zinc metalloprotein with three zinc finger structures.

摘要

从晚期精子细胞核中通过在Fractogel EMD SO3 - 650（M）上进行色谱分离以及在Nucleosil 300 7C18、二醇 - 120和Chemcosorb 3C18H上进行高效液相色谱法分离出猪完整过渡蛋白2（TP2）。圆二色光谱研究表明，TP2发生了微小但显著的锌依赖性二级结构变化。TP2具有三个潜在的锌指基序，通过原子吸收光谱法显示每分子蛋白质结合3个锌原子。这些结果连同TP2的氨基酸序列表明TP2是一种具有三个锌指结构的锌金属蛋白。