Bevec D, Hauber J
Novartis Research Institute, Department of Immunodermatology, Vienna, Austria.
Biol Signals. 1997 May-Jun;6(3):124-33. doi: 10.1159/000109118.
Eukaryotic initiation factor 5A (eIF-5A) is the only cellular protein known to contain the unusual amino acid hypusine, a modification that appears to be required for cell proliferation. This hypusine-modified protein stimulates synthesis of methionyl-puromycin in an in vitro assay which mimics the formation of the first peptide bond during protein synthesis, although the exact role of eIF-5A in vivo is still unknown. The unexpected finding that eIF-5A is a cellular cofactor of the HIV-1 Rev trans-activator protein may, however, provide a novel opportunity to reveal precisely what function eIF-5A performs in eukaryotic cells. In this review article, we first present a brief description of HIV-1 Rev function, followed by an overview of the data that identified eIF-5A as a Rev cofactor and, finally, discuss novel findings with respect to cellular eIF-5A activities.
真核生物起始因子5A（eIF-5A）是已知唯一含有罕见氨基酸hypusine的细胞蛋白，这种修饰似乎是细胞增殖所必需的。在模拟蛋白质合成过程中第一个肽键形成的体外试验中，这种hypusine修饰的蛋白能刺激甲硫氨酰-嘌呤霉素的合成，尽管eIF-5A在体内的确切作用仍不清楚。然而，eIF-5A是HIV-1 Rev反式激活蛋白的细胞辅因子这一意外发现，可能为精确揭示eIF-5A在真核细胞中发挥何种功能提供新机会。在这篇综述文章中，我们首先简要描述HIV-1 Rev的功能，接着概述将eIF-5A鉴定为Rev辅因子的数据，最后讨论关于细胞eIF-5A活性的新发现。
