Popović M, Popović N, Jovanova-Nesić K, Bokonjić D, Dobrić S, Kostić V S, Rosić N
Immunology Research Center Branislav Janković, Belgrade, FR Yugoslavia.
Int J Neurosci. 1997 Jun;90(1-2):87-97. doi: 10.3109/00207459709000628.
The present study was performed to investigate and compare the effect of acetylcholinesterase inhibitor, physostigmine (0.045, 0.060 and 0.075 mg/kg sc, 30 min before the tests) and Ca-antagonist, verapamil (1.0, 2.5, 5.0 and 10.0 mg/kg sc, 30 min before the tests), on two-way active avoidance (AA) learning (acquisition and performance) in nucleus basalis magnocellularis (NBM)-lesioned rats. Bilateral electrolytic lesions of NBM induced significant decrease of acquisition and performance of AA responses in rats. Physostigmine (0.060 mg/kg) significantly improved only acquisition of AA, while verapamil (2.5 and 5.0 mg/kg) significantly improved both type of AA behavior in NBM-lesioned rats. These results suggest that altered calcium homeostasis might play significant role in pathogenesis of experimental induced Alzheimer's disease (AD) and that administration of calcium antagonist such as verapamil might successfully ameliorate disturbances of learning and memory appeared after lesions of NBM.
本研究旨在调查并比较乙酰胆碱酯酶抑制剂毒扁豆碱（在测试前30分钟皮下注射，剂量分别为0.045、0.060和0.075毫克/千克）和钙拮抗剂维拉帕米（在测试前30分钟皮下注射，剂量分别为1.0、2.5、5.0和10.0毫克/千克）对基底大细胞核（NBM）损伤大鼠双向主动回避（AA）学习（习得和表现）的影响。NBM的双侧电解损伤导致大鼠AA反应的习得和表现显著下降。毒扁豆碱（0.060毫克/千克）仅显著改善了AA的习得，而维拉帕米（2.5和5.0毫克/千克）显著改善了NBM损伤大鼠的两种AA行为。这些结果表明，钙稳态改变可能在实验性诱导的阿尔茨海默病（AD）发病机制中起重要作用，并且给予钙拮抗剂如维拉帕米可能成功改善NBM损伤后出现的学习和记忆障碍。
