Petralia R S, Wang Y X, Singh S, Wu C, Shi L, Wei J, Wenthold R J
Laboratory of Neurochemistry, NIDCD/NIH, Bethesda, MD 20892-4162, USA.
J Chem Neuroanat. 1997 Jul;13(2):77-93. doi: 10.1016/s0891-0618(97)00023-9.
The metabotropic glutamate receptor, mGluR1 alpha, is postsynaptic in excitatory synapses in many populations of neurons and mediates long-term responses. The present study defines the distribution of this receptor using a new, highly specific monoclonal antibody to mGluR1 alpha. Overall distribution of immunostaining was similar to that described previously with polyclonal antibodies, including prominent staining in the olfactory bulb, interneurons of the CA1 hippocampus stratum oriens/alveus, globus pallidus, thalamus, Purkinje cells and in cells of the outer dorsal cochlear nucleus and with little or low staining in principal cells of the cerebral cortex and hippocampus. Interestingly, the well-known association of mGluR1 alpha receptors with neocortical interneurons was even more prevalent than previously noted with polyclonal antibodies. Ultrastructural studies in the hippocampus and cerebellum showed dense immunoperoxidase staining in postsynaptic membranes and densities and in perisynaptic and extrasynaptic membranes, as well as substantial cytoplasmic staining associated with organelles, especially the endoplasmic reticulum.
代谢型谷氨酸受体mGluR1α在许多神经元群体的兴奋性突触中位于突触后，并介导长期反应。本研究使用一种针对mGluR1α的新型高特异性单克隆抗体来确定该受体的分布。免疫染色的总体分布与先前用多克隆抗体描述的相似，包括嗅球、海马CA1海马原层/肺泡的中间神经元、苍白球、丘脑、浦肯野细胞以及外侧背侧耳蜗核细胞中有明显染色，而大脑皮层和海马的主要细胞中染色很少或很低。有趣的是，mGluR1α受体与新皮层中间神经元的著名关联比先前用多克隆抗体观察到的更为普遍。在海马体和小脑中的超微结构研究显示，突触后膜和致密物以及突触周围和突触外膜中有密集的免疫过氧化物酶染色，以及与细胞器特别是内质网相关的大量细胞质染色。
