Piskur J
Department of Genetics, Institute of Molecular Biology, University of Copenhagen, Denmark.
J Bacteriol. 1997 Sep;179(17):5614-7. doi: 10.1128/jb.179.17.5614-5617.1997.
A trans-acting element, MGT1 (also called CCE1), has previously been shown to be required in Saccharomyces cerevisiae for the preferential transmission of petite mitochondrial DNA (mtDNA) molecules over wild-type mtDNA molecules. In the present study a possible role of this nuclear gene in the transmission of mtDNA from various respiration-competent mutants was studied. Several of these mutants, lacking one or the other of two biologically active mitochondrial intergenic sequences, were employed in genetic crosses. When these deletion mutants were crossed to the parental wild-type strain in the MGT1/CCE1 background, the progeny contained predominantly wild-type mtDNA molecules. When crosses were performed in the mgt1/cce1 background, the parental molecules interacted in zygotes and underwent homologous recombination but wild-type and intergenic-deletion alleles were transmitted with equal frequencies.
一种反式作用元件MGT1(也称为CCE1),先前已证实在酿酒酵母中,相较于野生型线粒体DNA(mtDNA)分子,它对于小体型mtDNA分子的优先传递是必需的。在本研究中,对该核基因在来自各种呼吸能力正常的突变体的mtDNA传递中的可能作用进行了研究。这些突变体中有几个缺乏两个具有生物活性的线粒体基因间序列中的一个或另一个,被用于遗传杂交。当这些缺失突变体与MGT1/CCE1背景下的亲本野生型菌株杂交时,子代主要包含野生型mtDNA分子。当在mgt1/cce1背景下进行杂交时,亲本分子在合子中相互作用并进行同源重组,但野生型和基因间缺失等位基因以相等的频率传递。