来自科恩氏组分IV的人激肽原：抗原性与多种形式的比较。

Human kininogen from Cohns Fraction IV: comparisons of antigenicity and multiple forms.

作者信息

Hamberg U, Syvanen A C, Karkkainen T

出版信息

Adv Exp Med Biol. 1979;120B:173-83.

Abstract

Kininogen was isolated from Cohns fraction IV by DEAE-chromatography, gel filtration and ammonium sulphate precipitation. Immunologically pure kininogen was prepared by removal of protein impurities using specific immunoadsorbents with Sepharose-bound antibody. Anti-kininogen serum was raised in rabbits against the pure antigen. Comparison with anti-kininogen sera prepared with the biologically active LMW antigen from whole plasma suggested antigenic identity by double immunodiffusion analysis. The Cohn-kininogen was shown to contain mainly two components (85%) in about equal amounts focusing with peaks at pI 4.2 (42%) and pI 4.3 (43%). These represent apparently structurally altered forms of the native plasma kininogen focusing at pI 4.5-4.6 (54%), which occurred as a minor component (13%).

摘要

通过DEAE柱色谱法、凝胶过滤法和硫酸铵沉淀法从Cohn氏IV组分中分离出激肽原。使用与琼脂糖结合抗体的特异性免疫吸附剂去除蛋白质杂质，制备出免疫纯激肽原。用纯抗原在兔体内制备抗激肽原血清。通过双向免疫扩散分析，将其与用来自全血浆的具有生物活性的低分子量抗原制备的抗激肽原血清进行比较，结果表明二者抗原性相同。结果显示，Cohn氏激肽原主要含有两种含量大致相等的成分（85%），聚焦峰分别在pI 4.2（42%）和pI 4.3（43%）。这些显然代表了天然血浆激肽原聚焦于pI 4.5 - 4.6（54%）的结构改变形式，而天然血浆激肽原作为次要成分出现（13%）。