Z'Graggen K, Walz A, Mazzucchelli L, Strieter R M, Mueller C
Department of Pathology, University of Bem, Switzerland.
Gastroenterology. 1997 Sep;113(3):808-16. doi: 10.1016/s0016-5085(97)70175-6.
BACKGROUND & AIMS: Secretion of chemokines by epithelial cells may represent a crucial event in the pathogenesis of inflammatory bowel disease (IBD). Expression of the chemokine epithelial neutrophil-activating peptide 78 (ENA-78) was monitored in patients with IBD and normal controls.
In situ hybridizations were performed on 41 tissue specimens from 15 patients with IBD and 10 controls to detect ENA-78 messenger RNA (mRNA). Immunofluorescence stainings were used to localize ENA-78 protein.
Intestinal epithelial cells expressing ENA-78 mRNA at detectable levels are found at comparable frequencies in patients with Crohn's disease and ulcerative colitis. Tissue specimens with mild to moderate histological signs of disease activity show slightly higher frequencies of ENA-78 mRNA-expressing epithelial cells than areas with signs of severe disease activity (P = 0.14). Immunofluorescence stainings showed presence of the ENA-78 protein in > 90% of preserved epithelial cells in IBD, in control tissues, ENA-78 mRNA was not detectable, and ENA-78 protein was detectable in 0%-30% of epithelial cells.
The observations are in agreement with a role of the C-X-C chemokine ENA-78 in the pathogenesis of IBD.
上皮细胞分泌趋化因子可能是炎症性肠病（IBD）发病机制中的关键事件。本研究监测了IBD患者和正常对照者中趋化因子上皮中性粒细胞激活肽78（ENA-78）的表达情况。
对15例IBD患者和10例对照者的41份组织标本进行原位杂交，以检测ENA-78信使核糖核酸（mRNA）。采用免疫荧光染色法对ENA-78蛋白进行定位。
在克罗恩病和溃疡性结肠炎患者中，可检测到ENA-78 mRNA的肠上皮细胞出现频率相当。具有轻度至中度疾病活动组织学征象的组织标本中，表达ENA-78 mRNA的上皮细胞出现频率略高于具有重度疾病活动征象的区域（P = 0.14）。免疫荧光染色显示，IBD中超过90%的保存上皮细胞中存在ENA-78蛋白；在对照组织中，未检测到ENA-78 mRNA，且ENA-78蛋白在0%-30%的上皮细胞中可检测到。
这些观察结果表明C-X-C趋化因子ENA-78在IBD发病机制中发挥作用。
