Hyde K, Reid C J, Tebbutt S J, Weide L, Hollingsworth M A, Harris A
Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, England.
Gastroenterology. 1997 Sep;113(3):914-9. doi: 10.1016/s0016-5085(97)70187-2.
BACKGROUND & AIMS: The cystic fibrosis transmembrane conductance regulator (CFTR) protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. Pancreatic insufficiency is a feature of about 85% of patients with cystic fibrosis and is believed to be caused by pancreatic autolysis after pancreatic duct obstruction. The aim of this study was to investigate the expression of CFTR in the pancreas from early development to postnatal life to establish whether the CFTR plays a key role in development of the pancreatic duct epithelium.
Expression of CFTR from the start of the mid-trimester of human development through term to adult life by messenger RNA (mRNA) in situ hybridization was examined.
CFTR mRNA is detected throughout the pancreatic duct epithelium and its pattern of expression follows the differentiation of the duct system.
CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
囊性纤维化跨膜传导调节因子（CFTR）蛋白是一种小电导的腺苷3',5'-环磷酸（cAMP）激活的氯离子通道，存在于胰腺、气道、肠道、胆管、汗腺和男性生殖管道等上皮细胞的顶端膜中。胰腺功能不全是约85%囊性纤维化患者的一个特征，被认为是胰腺导管阻塞后胰腺自溶所致。本研究的目的是研究CFTR在胰腺从早期发育到出生后阶段的表达情况，以确定CFTR是否在胰腺导管上皮发育中起关键作用。
通过信使核糖核酸（mRNA）原位杂交检测从人类妊娠中期开始直至足月及成年期CFTR的表达。
在整个胰腺导管上皮中均检测到CFTR mRNA，其表达模式与导管系统的分化过程一致。
CFTR是人类胰腺导管细胞发育和分化的一个重要标志物。
