Takayama M, Yamane H, Konishi K, Iguchi H, Shibata S, Sunami K, Nakai Y, Nakagawa T
Department of Otolaryngology, Osaka City University Medical School, Japan.
Acta Otolaryngol Suppl. 1997;528:19-24.
Free radicals are known to cause damage to biological tissues. We used histochemical methods to examine the emergence of free radicals in kanamycin (KM)-treated guinea pig cochlea. As a preliminary in vitro study, the emergence of free radicals was observed in guinea pig cochlea incubated with KM. In an in vivo study, KM was placed on the guinea pig round window membrane and the emergence of free radicals was observed over time. The emergence of free radicals was observed along the luminal membrane and hair bundles of outer hair cells. The appearance and disappearance of free radicals in the cochlea coincided with the transport of KM into the inner ear. These findings suggest that the emergence of free radicals could be attributed to the administration of KM.
已知自由基会对生物组织造成损害。我们采用组织化学方法检测了卡那霉素（KM）处理的豚鼠耳蜗中自由基的出现情况。作为初步的体外研究，在与KM孵育的豚鼠耳蜗中观察到了自由基的出现。在体内研究中，将KM置于豚鼠圆窗膜上，并随时间观察自由基的出现情况。在外侧毛细胞的管腔膜和毛束上观察到了自由基的出现。耳蜗中自由基的出现和消失与KM进入内耳的过程一致。这些发现表明，自由基的出现可能归因于KM的给药。
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