运动训练和疲劳对骨骼肌线粒体及破碎肌浆网中45Ca++含量的影响。

Influence of exercise training and exhaustion on 45Ca++ content of skeletal muscle mitochondria and fragmented sarcoplasmic reticulum.

作者信息

Bonner H W, Buffington C K, Leslie S W

出版信息

Res Commun Chem Pathol Pharmacol. 1977 Dec;18(4):737-51.

Abstract

Following a 25 muCi/Kg i/p. injection, 45Ca++ activity from the mitochondria and fragmented sarcoplasmic reticulum (FSR) of the superficial rectus femoris (FG-fibers) and combined soleus muscle (SO-fibers) of trained (T, N = 9) and untrained (UT, N = 8) male Wistar rats was determined. The FG mitochondrial 45Ca++ activity from rested animals was 3.9 (T) and 5.3 (UT) times greater than the FSR from this muscle group while the so-mitochondrial 45Ca++ activity was 13.2 (T) and 20.5 (UT) times greater when compared with the respective SO-FSR (p less than .01). The mitochondrial 45Ca++ content of the SO-fibers from rested animals was 58% UT, p less than .01) and 148% (T, p less than .001) greater than that of the FG mitochondria. After 20-26 weeks of exercise training the SO-mitochondrial 45Ca++ content of the rested-T animals was approximately 18% lower (p less than .05) while the T-FG-mitochondrial 45Ca++ content was 48% less than that of UT animals (p less than .01) with a concomitant 28% greater 45Ca++ activity in SO-FSR for the T-group as compared to UT (p less than .01). Following a rapidly exhaustive treadmill run the mitochondrial 45Ca++ content in the UT group was depressed by 21% in SO-fibers and 37% in FG-fibers (p less than .01) with no changes occurring in the T-group. Exhaustion produced no significant changes in the 45Ca++ activity of the FSR from either group of animals.

摘要

对训练有素（T组，N = 9）和未训练（UT组，N = 8）的雄性Wistar大鼠，经腹腔注射25微居里/千克后，测定了股直肌浅层（FG纤维）和比目鱼肌联合肌（SO纤维）的线粒体及破碎肌浆网（FSR）中的45Ca++活性。静息动物的FG线粒体45Ca++活性比该肌群的FSR高3.9倍（T组）和5.3倍（UT组），而SO线粒体45Ca++活性与各自的SO - FSR相比高13.2倍（T组）和20.5倍（UT组）（p < 0.01）。静息动物SO纤维的线粒体45Ca++含量比FG线粒体高58%（UT组，p < 0.01）和148%（T组，p < 0.001）。经过20 - 26周的运动训练后，静息 - T组动物的SO线粒体45Ca++含量降低了约18%（p < 0.05），而T - FG线粒体45Ca++含量比UT组动物低48%（p < 0.01），与UT组相比，T组的SO - FSR中45Ca++活性增加了28%（p < 0.01）。在快速力竭的跑步机跑步后，UT组SO纤维中线粒体45Ca++含量降低了21%，FG纤维中降低了37%（p < 0.01），而T组没有变化。力竭对两组动物FSR的45Ca++活性均未产生显著影响。