Shingarova L N, Sagaĭdak L N, Berkova H P, Korobko V G
Bioorg Khim. 1997 May;23(5):428-33.
A number of hybrid proteins containing amino acid sequences of human lymphotoxin and human and mouse tumor necrosis factors were obtained by means of genetic engineering. By using these proteins, an antigenic determinant in the molecule of human tumor necrosis factor for monoclonal antibodies E7H2 (MAb E7H2), previously developed against this factor was localized. It was demonstrated by Western blot analysis that the MAb E7H2-binding site is located in the sequence region 37-49 of human tumor necrosis factor and includes the sequence Val41GluLeuArg44 directly interacting with MAb E7H2.
通过基因工程获得了多种含有人类淋巴毒素以及人类和小鼠肿瘤坏死因子氨基酸序列的杂合蛋白。利用这些蛋白，定位了先前针对人类肿瘤坏死因子研制的单克隆抗体E7H2（MAb E7H2）在该因子分子中的一个抗原决定簇。蛋白质免疫印迹分析表明，MAb E7H2结合位点位于人类肿瘤坏死因子的37 - 49序列区域，且包含与MAb E7H2直接相互作用的Val41GluLeuArg44序列。
