Sugiura T, Tashiro T, Yamamori H, Morishima Y, Otsubo Y, Hayashi N, Furukawa K, Nitta H, Nakajima N, Ishizuka T, Tatibana M, Ino H, Ito I
First Department of Surgery, Chiba University School of Medicine, Japan.
Nutrition. 1997 Sep;13(9):783-7. doi: 10.1016/s0899-9007(97)00189-5.
The purpose of this study was to investigate the effect of insulin-like growth factor-1 (IGF-1) on the translocation of endotoxin from the gut of burned rats. Twenty-eight male Sprague-Dawley rats (7-wk-old) were subjected to 20% full-thickness scald burns and were divided into two groups. One group received 4 mg.kg-1.d-1 of IGF-1 (IGF-1 group, n = 14), and the other received saline (control group, n = 13). All rats were fed exclusively by total parenteral nutrition (TPN). On the second postburn day, rats were killed. The amount of endotoxin in the liver and spleen were measured. RNA from the terminal ileum was extracted, and Northern blot analyses of alpha-tubulin, beta-actin, cell division cycle-2 (cdc2), and immunoglobulin-A (IgA) were performed. Nitrogen balance was improved (p < 0.001), and the wet weight of intestine and its mucosa were increased significantly in the burned rats that received IGF-1. Gene expression of alpha-tubulin and beta-actin were not changed. Cdc2 was elevated (P < 0.05), but IgA was decreased (P < 0.05) in the IGF-1 group. Levels of endotoxin in the liver and spleen were significantly reduced (P<0.05) by the administration of IGF-1. A negative correlation between the levels of endotoxin in the liver and the weight of the intestinal mucosa was observed. In conclusion, IGF-1 improved nitrogen balance, promoted the proliferation of intestinal mucosa and reduced the translocation of endotoxin.
本研究的目的是探讨胰岛素样生长因子-1（IGF-1）对烧伤大鼠肠道内毒素移位的影响。28只雄性Sprague-Dawley大鼠（7周龄）接受20%全层烫伤，并分为两组。一组接受4mg·kg-1·d-1的IGF-1（IGF-1组，n = 14），另一组接受生理盐水（对照组，n = 13）。所有大鼠均通过全胃肠外营养（TPN）单独喂养。在烧伤后第二天，处死大鼠。测量肝脏和脾脏中的内毒素含量。提取回肠末端的RNA，并进行α-微管蛋白、β-肌动蛋白、细胞分裂周期2（cdc2）和免疫球蛋白A（IgA）的Northern印迹分析。接受IGF-1的烧伤大鼠氮平衡得到改善（p < 0.001），肠道及其黏膜的湿重显著增加。α-微管蛋白和β-肌动蛋白的基因表达未改变。IGF-1组中cdc2升高（P < 0.05），但IgA降低（P < 0.05）。给予IGF-1后，肝脏和脾脏中的内毒素水平显著降低（P<0.05）。观察到肝脏内毒素水平与肠黏膜重量之间呈负相关。总之，IGF-1改善了氮平衡，促进了肠黏膜增殖并减少了内毒素移位。
